An in Vitro Approach for Interspecies Extrapolation Using Animal and Human Airway Epithelial Cell Culture
An in Vitro Approach for Interspecies Extrapolation Using Animal and Human Airway Epithelial Cell Culture
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利用动物和人类气道上皮细胞培养进行种间外推的体外方法
DOI：
10.1007/978-1-4684-5625-7_19
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发表时间：
1989
影响因子：
2.4
通讯作者：
M. Straub
中科院分区：
文献类型：
--
作者：
M. Emura;M. Riebe;U. Mohr;J. Jacob;G. Grimmer;J. Wen;M. Straub
为了评估癌症发展的风险，我们通常需要将实验室实验的数据推断到人类的情况。然而，对不同哺乳动物物种的研究得出了各种各样的定性和定量结果。仅举几个例子，将苯并（a）芘（BaP）与来自呼吸器官的微粒体制剂一起孵育，在大鼠和恒河猴中主要产生反式4,5-二氢-4,5-二羟基苯并（a）芘（反式4,5-二醇），而在叙利亚仓鼠和人类中则产生反式7,8-二醇（参见Cohen和Ashurst的评论，1983）。多环芳烃 (PAH) 和香烟烟雾会诱发大鼠肺周围区域、人类肺门区域和叙利亚仓鼠上呼吸道的呼吸器官肿瘤（参见 Mohr 和 Dungworth 的评论，1988 年）。在呼吸道细胞培养物中，12-0-十四烷酰佛波醇-13-乙酸酯 (TPA) 增强大鼠细胞的集落形成，而对仓鼠和人类细胞具有抑制作用（Beeman 等，1987）。因此，我们应该认识到这种与物种相关的变异，并尝试了解其发生的机制，以使推断更加准确。
To assess the risk of cancer development we usually need to extrapolate data from laboratory experiments to the human situation. There is, however, a wide variety of both qualitative and quantitative results from studies with different mammalian species. To cite but a few examples, incubation of benzo(a)pyrene (BaP) with the microsomal preparations from respiratory organs produced predominantly trans4,5-dihydro-4,5-dihydroxybenzo(a)pyrene (trans-4,5-diol) in rats and rhesus monkeys, while in Syrian hamsters and humans trans-7,8-diol was produced (see review by Cohen and Ashurst, 1983). Polycyclic aromatic hydrocarbons (PAHs) and cigarette smoke induce tumors of the respiratory organs in the pulmonary peripheral region in rats, in the pulmonary hilar region in humans and in the upper respiratory tract in Syrian hamsters (see review by Mohr and Dungworth, 1988). In the respiratory tract cell cultures, 12-0-tetradecanoylphorbol-13-acetate (TPA) enhanced colony formation of rat cells, while it was inhibitory to hamster and human cells (Beeman et al., 1987). We should, therefore, recognize such species-dependent variations and try to understand the mechanisms underlying their occurrence to make the extrapolation more accurate.
影响因子：
2.2
作者：
Yan Z;Wang H;Zheng WQ;Hu ZD
通讯作者：
Hu ZD