Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
Conservation and rewiring of functional modules revealed by an epistasis map in fission yeast.
复制标题
DOI：
10.1126/science.1162609
复制
发表时间：
2008-10-17
期刊：
影响因子：
--
通讯作者：
Krogan NJ
中科院分区：
文献类型：
--
作者：
Roguev A;Bandyopadhyay S;Zofall M;Zhang K;Fischer T;Collins SR;Qu H;Shales M;Park HO;Hayles J;Hoe KL;Kim DU;Ideker T;Grewal SI;Weissman JS;Krogan NJ
通过系统地测量与突变对相关的表型，构建了裂殖酵母裂殖酵母的上位性图谱（E-MAP）。这张高密度、定量的遗传相互作用图谱集中在染色体功能的各个方面，包括转录调控和DNA修复/复制。E-MAP发现了RNA干扰（RNAi）机制（rsh 1）的一个先前未识别的组分，并将RNAi途径与其他几个生物过程联系起来。S. pombe E-MAP与芽殖酵母的类似遗传图谱的比较显示，尽管参与相似生物过程的基因之间的负相互作用是保守的，但编码物理相关蛋白质的基因对之间的正相互作用和总体遗传谱甚至更加保守。因此，保守发生在功能模块（蛋白质复合物）的水平上，但模块之间的遗传串扰可能有很大的不同。
An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway to several other biological processes. Comparison of the S. pombe E-MAP to an analogous genetic map from the budding yeast revealed that, whereas negative interactions were conserved between genes involved in similar biological processes, positive interactions and overall genetic profiles between pairs of genes coding for physically associated proteins were even more conserved. Hence, conservation occurs at the level of the functional module (protein complex), but the genetic cross talk between modules can differ substantially.
登录
查看更多内容
影响因子：
5.3
作者：
Millband, DN;Hardwick, KG
通讯作者：
Hardwick, KG
影响因子：
64.8
作者：
Gavin, AC;Bösche, M;Superti-Furga, G
通讯作者：
Superti-Furga, G
影响因子：
4.8
作者：
Dul, Barbara E.;Walworth, Nancy C.
通讯作者：
Walworth, Nancy C.
DOI：
10.1073/pnas.232688099
发表时间：
2003-01-07
影响因子：
11.1
作者：
Hall, IM;Noma, K;Grewal, SIS
通讯作者：
Grewal, SIS
影响因子：
16
作者：
Otero, G;Fellows, J;Svejstrup, JQ
通讯作者：
Svejstrup, JQ