Improved MALDI-TOF imaging yields increased protein signals at high molecular mass.
Improved MALDI-TOF imaging yields increased protein signals at high molecular mass.
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DOI：
10.1016/j.jasms.2008.09.008
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发表时间：
2009-01
影响因子：
3.2
通讯作者：
Lau SS
中科院分区：
文献类型：
--
作者：
Leinweber BD;Tsaprailis G;Monks TJ;Lau SS
Matrix assisted laser desorption ionization (MALDI) mass spectrum images are created from an array of mass spectra collected over a tissue surface. We have increased the mass range of proteins that can be detected in tissue sections from kidneys of different rodent species, by a modification of the sandwich technique which involves co-crystallizing matrix with analyte. A tissue section is placed upon a drop of sinapinic acid matrix dissolved in 90% ethanol and 0.5% Triton X-100. Once the matrix has dried, a seed layer of sinapinic crystals is added as a dispersion in xylene. Additional layers of sinapinic acid are added as solutions in 90% ethanol followed by 50% acetonitrile. Numerous peaks with signal to noise ratio of four or greater are observed between 25 kDa to 50 kDa. This represents approximately 10 times as many peaks as are detected using traditional matrix spotting and spraying.
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