Fine mapping of a male sterility gene, vr1, on chromosome 4 in rice
Fine mapping of a male sterility gene, vr1, on chromosome 4 in rice
复制标题
水稻 4 号染色体上雄性不育基因 vr1 的精细定位
DOI：
10.1007/s11032-010-9472-7
复制
发表时间：
2011-08
影响因子：
3.1
通讯作者：
Qiming Deng
中科院分区：
文献类型：
--
作者：
Jin Zhang;Lei Ding;Lingxia Wang;Ming He;Manhua Zhang,;Aiping Zheng;Ping Li;Mingguang Chu;Shuangcheng Li;Huainian Liu;Jun Zhu;Shiquan Wang;Qiming Deng
摘要xs1是一个水稻雄性不育突变体。xs1突变体的花粉发育正常，直到空泡化阶段，此时xs1花粉不能空泡化，不能产生活花粉。遗传分析表明，xs1突变体表型受一个定位于水稻4号染色体上的单隐性基因vr1控制。为了精确定位vr1位点，我们建立了两个大的定位群体，并从公开的水稻基因组序列中开发了多个SSR和InDel标记。通过染色体行走策略，vr1基因最终被定位在0.27 cM的遗传间隔内，位于标记FID30和FS15的两侧，距离分别为0.11和0.16 cM，并与标记FC4-2共分离。根据粳稻基因组序列，vr1位点估计覆盖了一个48kb的区域，其中包含8个可能的基因。本研究结果将为vr1基因的克隆和功能鉴定提供依据。
Abstractxs1 is a male sterile rice mutant derived from a spontaneous mutation. Pollen development in the xs1 mutant proceeds normally until the vacuolation stage, at which time xs1 pollen fails to vacuolate and no viable pollen is produced. Genetic analysis indicates that the xs1 mutant phenotype is controlled by a single recessive gene, designated vacuolation retardation 1 (vr1), which was mapped to rice chromosome 4. In order to fine-map the vr1 locus, two large mapping populations were generated and several SSR and InDel markers were developed from publicly available rice genomic sequences. By employing a strategy of chromosome-walking, the vr1 gene was finally located within a genetic interval of 0.27 cM, flanked by the markers FID30 and FS15, with distances of 0.11 and 0.16 cM, respectively, and co-segregating with the marker FC4-2. Based on the japonica rice genome sequence, the vr1 locus is estimated to cover a 48-kb region containing eight putative genes. Our results will facilitate the cloning and functional characterization of the vr1 gene.
登录
查看更多内容
影响因子：
5.1
作者：
Liangran Zhang;Jiayi Tao;Shunxin Wang;K. Chong;Tai Wang
通讯作者：
Liangran Zhang;Jiayi Tao;Shunxin Wang;K. Chong;Tai Wang
影响因子：
5.4
作者：
Chen, X;Temnykh, S;McCouch, SR
通讯作者：
McCouch, SR
影响因子：
5.1
作者：
Jiang, S;Cai, M;Ramachandran, S
通讯作者：
Ramachandran, S
影响因子：
3.1
作者：
Nonomura, KI;Nakano, M;Kurata, N
通讯作者：
Kurata, N
影响因子：
11.4
作者：
Li, SC;Yang, L;Li, P
通讯作者：
Li, P