Describing two-photon absorptivity of fluorescent proteins with a new vibronic coupling mechanism.
Describing two-photon absorptivity of fluorescent proteins with a new vibronic coupling mechanism.
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DOI：
10.1021/jp211020k
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发表时间：
2012-02-09
影响因子：
3.3
通讯作者：
Rebane, A.
中科院分区：
文献类型：
--
作者：
Drobizhev, M.;Makarov, N. S.;Tillo, S. E.;Hughes, T. E.;Rebane, A.
荧光蛋白（FP）作为基因编码探针广泛应用于双光子显微镜中。因此，了解其双光子吸收（2PA）特性的物理基础对于创建双光子更亮的突变体至关重要。另一方面，它可以让我们更好地了解 FP 生色团与复杂蛋白质环境的分子相互作用。据了解，与纯电子跃迁最强的单光子吸收光谱相比，许多FP的2PA谱以电子跃迁为主。这种强度重新分布的物理机制尚不清楚。在这里，我们提出了一个新的物理模型，通过“赫兹伯格-特勒”型振动耦合基态和激发态的永久偶极矩（Δμ）与键长交替坐标之间的差异来解释这种效应。该模型还使我们能够通过“Herzberg-Teller”项和 Franck-Condon 项之间的干扰定量描述具有相同发色团的一系列红色 FP 中 2PA 峰强度的较大变化。
Fluorescent proteins (FPs) are widely used in two-photon microscopy as genetically-encoded probes. Understanding the physical basics of their two-photon absorption (2PA) properties is therefore crucial for creation of two-photon brighter mutants. On the other hand, it can give us better insight into molecular interactions of the FP chromophore with complex protein environment. It is known that, compared to one-photon absorption spectrum, where the pure electronic transition is the strongest, the 2PA spectrum of a number of FPs is dominated by a vibronic transition. The physical mechanism of such intensity redistribution is not understood. Here we present a new physical model that explains this effect through the “Herzberg-Teller”-type vibronic coupling of the difference between the permanent dipole moments in the ground and excited states (Δμ) to the bond-length-alternating coordinate. This model also enables us to quantitatively describe a large variability of the 2PA peak intensity in a series of red FPs with the same chromophore through the interference between the “Herzberg-Teller” and Franck-Condon terms.
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影响因子：
1.9
作者：
Hashimoto, Hiroshi;Isobe, Keisuke;Midorikawa, Katsumi
通讯作者：
Midorikawa, Katsumi
影响因子：
56.9
作者：
Hales, Joel M.;Matichak, Jonathan;Marder, Seth R.
通讯作者：
Marder, Seth R.
影响因子：
3.3
作者：
Drobizhev, M.;Makarov, N. S.;Rebane, A.
通讯作者：
Rebane, A.
影响因子：
4.3
作者：
Cogné-Laage, E;Allemand, JF;Jullien, L
通讯作者：
Jullien, L
影响因子：
2.8
作者：
CALLIS, PR
通讯作者：
CALLIS, PR