A closer look at neuron interaction with track-etched microporous membranes.
A closer look at neuron interaction with track-etched microporous membranes.
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DOI：
10.1038/s41598-018-33710-6
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发表时间：
2018-10-19
影响因子：
4.6
通讯作者：
Ye H
中科院分区：
文献类型：
--
作者：
George JH;Nagel D;Waller S;Hill E;Parri HR;Coleman MD;Cui Z;Ye H
微孔膜支持神经突生长进入并穿过微通道，为传统培养皿提供不同类型的神经生长平台。微孔膜用于支持各种类型的培养物，然而，孔径与神经突通过膜生长的关系尚未得到很好的表征。在这项研究中，人类细胞系（SH-SY5Y）被分化为神经元样细胞，并在径迹蚀刻的微孔膜上培养，孔径和通道直径选择适应神经突宽度（0.8μm至5μm）。虽然神经突延伸穿过所有孔径，但培养 5 天后，发现膜非种子侧的神经突覆盖范围与通道直径成正比。当神经培养物不汇合时，通过膜孔的神经突生长显着减少。扫描电子显微镜显示，神经突桥接毛孔并环绕毛孔边缘，从而降低了神经突进入毛孔通道的总体可能性。这些发现强调了孔径、细胞片汇合和接触引导在引导神经突通过孔生长中的作用，并且可能在寻求使用物理基质来维持单独的神经群体同时允许培养物之间的神经突接触的应用中有用。
Microporous membranes support the growth of neurites into and through micro-channels, providing a different type of neural growth platform to conventional dish cultures. Microporous membranes are used to support various types of culture, however, the role of pore diameter in relation to neurite growth through the membrane has not been well characterised. In this study, the human cell line (SH-SY5Y) was differentiated into neuron-like cells and cultured on track-etched microporous membranes with pore and channel diameters selected to accommodate neurite width (0.8 µm to 5 µm). Whilst neurites extended through all pore diameters, the extent of neurite coverage on the non-seeded side of the membranes after 5 days in culture was found to be directly proportional to channel diameter. Neurite growth through membrane pores reduced significantly when neural cultures were non-confluent. Scanning electron microscopy revealed that neurites bridged pores and circumnavigated pore edges – such that the overall likelihood of a neurite entering a pore channel was decreased. These findings highlight the role of pore diameter, cell sheet confluence and contact guidance in directing neurite growth through pores and may be useful in applications that seek to use physical substrates to maintain separate neural populations whilst permitting neurite contact between cultures.
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影响因子：
14
作者：
Johansson, F;Carlberg, P;Kanje, M
通讯作者：
Kanje, M
DOI：
10.1002/jbm.b.32656
发表时间：
2012-05-01
影响因子：
3.4
作者：
Reich, Uta;Fadeeva, Elena;Reuter, Guenter
通讯作者：
Reuter, Guenter
影响因子：
2.7
作者：
Smit, M;Leng, J;Klemke, RL
通讯作者：
Klemke, RL
影响因子：
14
作者：
Ferrari, Aldo;Cecchini, Marco;Beltram, Fabio
通讯作者：
Beltram, Fabio
使用 3D 配置的微流控芯片模拟肝窦结构和功能
影响因子：
6.1
作者：
Du, Yu;Li, Ning;Long, Mian
通讯作者：
Long, Mian