Chemical & RNAi screening at MSKCC: a collaborative platform to discover & repurpose drugs to fight disease.
Chemical & RNAi screening at MSKCC: a collaborative platform to discover & repurpose drugs to fight disease.
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化学
DOI：
--
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发表时间：
2014
影响因子：
1.8
通讯作者：
Hakim Djaballah
中科院分区：
文献类型：
--
作者：
B. Bhinder;C. Antczak;David Shum;C. Radu;Jeni P. Mahida;Nancy Liu;G. Ibáñez;Balajee Somalinga Raja;P. Calder;Hakim Djaballah
纪念斯隆凯特琳癌症中心（MSKCC）已经实施了一个全面服务的最先进的高通量筛选核心设施（HTSCF）的创建，配备了现代机器人和定制的筛选数据管理资源，以快速存储和查询化学和RNAi筛选数据输出。该设施的使命是为肿瘤临床医生和研究人员提供具有成本效益的HTS解决方案，用于化学和RNAi筛选，最终目标是新靶点识别和药物发现。HTSCF成立于2003年，旨在支持该机构在分子药理学和治疗药物领域的发展，以对抗癌症等慢性疾病。这项工作需要广泛的技术开发专业知识来建立稳健和创新的分析方法，大量收集不同的化学和RNAi双链物来探测特定的细胞事件，复杂的化合物和数据处理能力，以及在分析开发，击中验证和表征方面的深厚知识。我们的目标是不断创新，我们坚定地相信将传统的药物发现模式转变为现在的转化研究，并考虑到患者未满足的临床需求。当通过原发性HTS鉴定地高辛用于临床治疗Vb期视网膜母细胞瘤时，我们重新利用fda批准的药物的努力取得了成果。总之，我们设施的总体目标是识别新的化学探针，研究与研究者在化学生物学和功能基因组学方面的研究兴趣相关的细胞过程，并有助于加速学术界的药物发现过程。
Memorial Sloan Kettering Cancer Center (MSKCC) has implemented the creation of a full service state-of-the-art High-throughput Screening Core Facility (HTSCF) equipped with modern robotics and custom-built screening data management resources to rapidly store and query chemical and RNAi screening data outputs. The mission of the facility is to provide oncology clinicians and researchers alike with access to cost-effective HTS solutions for both chemical and RNAi screening, with an ultimate goal of novel target identification and drug discovery. HTSCF was established in 2003 to support the institution's commitment to growth in molecular pharmacology and in the realm of therapeutic agents to fight chronic diseases such as cancer. This endeavor required broad range of expertise in technology development to establish robust and innovative assays, large collections of diverse chemical and RNAi duplexes to probe specific cellular events, sophisticated compound and data handling capabilities, and a profound knowledge in assay development, hit validation, and characterization. Our goal has been to strive for constant innovation, and we strongly believe in shifting the paradigm from traditional drug discovery towards translational research now, making allowance for unmet clinical needs in patients. Our efforts towards repurposing FDA-approved drugs fructified when digoxin, identified through primary HTS, was administered in the clinic for treatment of stage Vb retinoblastoma. In summary, the overall aim of our facility is to identify novel chemical probes, to study cellular processes relevant to investigator's research interest in chemical biology and functional genomics, and to be instrumental in accelerating the process of drug discovery in academia.
DOI：
10.2174/138620712800194468
发表时间：
2012-06-01
影响因子：
1.8
作者：
Ibanez G;Shum D;Blum G;Bhinder B;Radu C;Antczak C;Luo M;Djaballah H
通讯作者：
Djaballah H
影响因子：
16
作者：
Feldman, Taya;Kabaleeswaran, Venkataraman;Jang, Se Bok;Antczak, Christophe;Djaballah, Hakim;Wu, Hao;Jiang, Xuejun
通讯作者：
Jiang, Xuejun