MUC5B glycosylation in human saliva reflects blood group and secretor status.
MUC5B glycosylation in human saliva reflects blood group and secretor status.
复制标题
人类唾液中的 MUC5B 糖基化反映了血型和分泌者状态。
作者：
K. Thomsson;B. Schulz;N. Packer;N. Karlsson
本研究旨在表征人类唾液糖型和携带高分子量糖蛋白组分MG 1的主要ABO血型的天然糖基化变异，MG 1主要由MUC 5 B粘蛋白组成。用十二烷基硫酸钠-琼脂糖/聚丙烯酰胺复合凝胶电泳（SDS-AgPAGE）纯化A、B和O型血个体的还原和烷基化粘蛋白，将其印迹到聚偏氟乙烯（PVDF）膜上，并用阿辛蓝显色。通过还原性β-消除从MUC 5 B糖型条带中释放O-连接寡糖，并通过液相色谱（LC）和电喷雾离子阱质谱（MS）进行分析。发现慢迁移MUC 5 B组分（sm）主要由中性寡糖组成，而快迁移（fm）组分主要由硫酸化寡糖组成。在所有糖型上发现ABO血型特异性序列，并对含有血型A和B型序列的新型寡糖进行测序。这是第一次从分子水平描述血型ABO系统对唾液MUC 5 B寡糖的影响。将这些结果从A、B和O三个个体扩展到更大的群体（29个个体）中，我们在23个个体的MUC 5 B上发现了对应于供体血型的寡糖序列。其余6个个体的特征为高度唾液酸化。这些个体被指定为非分泌型，而血型表达的个体被指定为分泌型。用抗体进行的蛋白质印迹分析证实非分泌细胞中Sialyl刘易斯a（Si-Le（a））的表达增加。我们的研究结果强调，唾液MUC 5 B由具有不同糖基化的糖型组成，这些糖型在个体之间差异很大，其中一些变化是由于血型和分泌状态。
This study aimed to characterize human salivary glycoforms and the natural glycosylation variation of the major ABO blood group bearing high molecular weight glycoprotein fraction MG1, which mainly consists of MUC5B mucin. Reduced and alkylated mucins from individuals of blood group A, B, and O were purified by sodium dodecyl sulfate-agarose/polyacrylamide composite gel electrophoresis (SDS-AgPAGE), blotted to polyvinylidene fluoride (PVDF) membranes, and visualized with alcian blue. O-linked oligosaccharides were released from MUC5B glycoform bands by reductive beta-elimination and analyzed by liquid chromatography (LC) electrospray ion trap mass spectrometry (MS). Slow electrophoretically migrating MUC5B components (sm) were found to be dominated by neutral oligosaccharides, and fast-migrating (fm) components were dominated by sulfated oligosaccharides. ABO blood group-specific sequences were found on all glycoforms, and novel oligosaccharides containing blood group A and B type sequences were sequenced. This is the first molecular description of the influence of the blood group ABO system on salivary MUC5B oligosaccharides. Expanding these results from the three A, B, and O individuals into larger population (29 individuals), we found oligosaccharide sequences corresponding to the blood group of the donor on MUC5B from 23 individuals. The remaining six individuals were characterized by a high degree of sialylation. These individuals were assigned as nonsecretors, whereas blood group-expressing individuals were assigned as secretors. Western blot assays with antibodies confirmed increased expression of Sialyl Lewis a (Si-Le(a)) in the nonsecretors. Our results highlight that salivary MUC5B consists of glycoforms with distinct glycosylation that vary extensively between individuals and that some of this variation is owing to blood group and secretor status.
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影响因子：
2.9
作者：
A. Prakobphol;K. Thomsson;Gunnar C. Hansson;Steven D. Rosen;M. Singer;Nancy J. Phillips;K. Medzihradszky;Alma L. Burlingame;Hakon Leffler;Susan J. Fisher
通讯作者：
A. Prakobphol;K. Thomsson;Gunnar C. Hansson;Steven D. Rosen;M. Singer;Nancy J. Phillips;K. Medzihradszky;Alma L. Burlingame;Hakon Leffler;Susan J. Fisher
DOI：
--
发表时间：
1993-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Bobek;Hsiaoyun Tsai;A. R. Biesbrock;Michael J. Levine
通讯作者：
L. Bobek;Hsiaoyun Tsai;A. R. Biesbrock;Michael J. Levine
影响因子：
4.1
作者：
RAMASUBBU, N;REDDY, MS;LEVINE, MJ
通讯作者：
LEVINE, MJ
高分子量人粘蛋白是 ABH、Le(a) 和 Le(b) 血型抗原的主要唾液载体。
DOI：
10.1177/10454411930040031001
发表时间：
1993
期刊：
Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists
影响因子：
--
作者：
Prakobphol,A;Leffler,H;Fisher,SJ
通讯作者：
Fisher,SJ