Blockade of adrenoreceptors inhibits the splenic response to stroke.
Blockade of adrenoreceptors inhibits the splenic response to stroke.
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DOI：
10.1016/j.expneurol.2009.03.044
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发表时间：
2009-07
影响因子：
5.3
通讯作者：
Pennypacker, Keith R.
中科院分区：
文献类型：
--
作者：
Ajmo, Craig T., Jr.;Collier, Lisa A.;Leonardo, Christopher C.;Hall, Aaron A.;Green, Suzanne M.;Womble, Tracy A.;Cuevas, Javier;Willing, Alison E.;Pennypacker, Keith R.
最近的研究强调了外周免疫系统在卒中后迟发性细胞变性中的作用。永久性大脑中动脉闭塞（MCAO）脑卒中模型中，脾脏体积减小。这种减少是通过脾免疫细胞的释放发生的。脑卒中后24小时用人脐带血细胞（HUCBC）进行全身治疗可阻断脾脏大小的减小，同时显著减少梗死体积。脾切除术在MCAO前两周也减少了梗死体积，进一步证明了该器官在中风诱导的神经变性中的有害作用。MCAO后交感神经系统的激活导致儿茶酚胺水平升高，通过脾神经直接支配，以及从肾上腺髓质释放后整个体循环。这些儿茶酚胺结合脾α和β肾上腺素受体。本研究探讨儿茶酚胺是否调节脑卒中脾反应。雄性Sprague-Dawley大鼠在MCAO前两周接受脾去神经或注射卡维地洛（一种泛肾上腺素受体阻滞剂）、普拉唑嗪（一种α1受体阻滞剂）或心得安（一种β受体阻滞剂）。脾酪氨酸羟化酶表达减少证实神经失支配。MCAO前去神经支配未改变梗死体积或脾脏大小。心得安治疗对这些结果也没有影响。用哌唑嗪或卡维地洛治疗均可防止脾体积缩小，但只有卡维地洛能显著减少梗死体积（p<0.05）。这些结果表明，循环血液中的儿茶酚胺通过激活α和β肾上腺素能受体来调节脾对中风的反应。
Recent studies have highlighted the involvement of the peripheral immune system in delayed cellular degeneration after stroke. In the permanent middle cerebral artery occlusion (MCAO) model of stroke, the spleen decreases in size. This reduction occurs through the release of splenic immune cells. Systemic treatment with human umbilical cord blood cells (HUCBC) 24 hours post-stroke blocks the reduction in spleen size while significantly reducing infarct volume. Splenectomy two weeks prior to MCAO also reduces infarct volume, further demonstrating the detrimental role of this organ in stroke-induced neurodegeneration. Activation of the sympathetic nervous system after MCAO results in elevated catecholamine levels both at the level of the spleen, through direct splenic innervation, and throughout the systemic circulation upon release from the adrenal medulla. These catecholamines bind to splenic α and β adrenoreceptors. This study examines whether catecholamines regulate the splenic response to stroke. Male Sprague-Dawley rats either underwent splenic denervation two weeks prior to MCAO or received injections of carvedilol, a pan adrenergic receptor blocker, prazosin, an α1 receptor blocker, or propranolol, a β receptor blocker. Denervation was confirmed by reduced splenic expression of tyrosine hydroxylase. Denervation prior to MCAO did not alter infarct volume or spleen size. Propranolol treatment also had no effects on these outcomes. Treatment with either prazosin or carvedilol prevented the reduction in spleen size, yet only carvedilol significantly reduced infarct volume (p<0.05). These results demonstrate that circulating blood borne catecholamines regulate the splenic response to stroke through the activation of both α and β adrenergic receptors.
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影响因子：
2.9
作者：
Duckworth, EAM;Butler, TL;Pennypacker, KR
通讯作者：
Pennypacker, KR
影响因子：
3.1
作者：
Barone, Frank C.;Willette, Robert N.;Coatney, Robert W.
通讯作者：
Coatney, Robert W.
影响因子：
3.8
作者：
Cardoso, Leonardo Maximo;Colombari, Debora Simoes de Almeida;Colombari, Eduardo
通讯作者：
Colombari, Eduardo
影响因子：
2.5
作者：
Lorton, D;Lubahn, C;Bellinger, DL
通讯作者：
Bellinger, DL
影响因子：
8.3
作者：
KORPELAINEN, JT;SOTANIEMI, KA;MYLLYLA, VV
通讯作者：
MYLLYLA, VV