Analysis of anti-rotavirus activity of extract from Stevia rebaudiana.
Analysis of anti-rotavirus activity of extract from Stevia rebaudiana.
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甜叶菊提取物的抗轮状病毒活性分析。
DOI：
10.1016/s0166-3542(00)00134-0
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发表时间：
2001
影响因子：
7.6
通讯作者：
S. Shigeta
中科院分区：
文献类型：
--
作者：
Kazuo Takahashi;M. Matsuda;Kazutaka Ohashi;Koki Taniguchi;Osamu Nakagomi;Yurika Abe;S. Mori;Naohiko Sato;Koichi Okutani;S. Shigeta
研究了甜菊热水提取物的抗人轮状病毒（HRV）活性。SE在体外抑制所有四种血清型HRV的复制。SE的这种抑制作用并没有减少对先前暴露的SE盐酸在pH 2的30分钟。用放射性标记的纯化病毒进行的结合试验表明，SE的抑制机制是阻断病毒结合。SE抑制抗VP7单克隆抗体与HRV感染的MA104细胞的结合。SE的抑菌成分是具有不同离子电荷的异质阴离子多糖。成分分析表明，甜菊糖抑制活性最高的纯化组分为相对分子质量为9800的阴离子多糖，含有丝氨酸和丙氨酸等氨基酸。糖残基分析表明糖醛酸是糖组分。它不含氨基和中性糖和硫酸盐残基。这些发现表明SE可能与37 kD VP 7结合，并通过空间位阻干扰VP 7与细胞受体的结合，从而导致病毒附着细胞的阻断。
Anti-human rotavirus (HRV) activity of hot water extracts from Stevia rebaudiana (SE) was examined. SE inhibited the replication of all four serotypes of HRV in vitro. This inhibitory effect of SE was not reduced on the prior exposure of SE to HCl for 30 min at pH 2. Binding assay with radiolabeled purified viruses indicated that the inhibitory mechanism of SE is the blockade of virus binding. The SE inhibited the binding of anti-VP7 monoclonal antibody to HRV-infected MA104 cells. The inhibitory components of SE were found to be heterogeneous anionic polysaccharides with different ion charges. The component analyses suggested that the purified fraction named as Stevian with the highest inhibitory activity consists of the anionic polysaccharide with molecular weight of 9800, and contains Ser and Ala as amino acids. Analyses of sugar residues suggest uronic acid(s) as sugar components. It did not contain amino and neutral sugars and sulfate residues. These findings suggest that SE may bind to 37 kD VP7 and interfere with the binding of VP7 to the cellular receptors by steric hindrance, which results in the blockade of the virus attachment to cells.
DOI：
10.1172/jci116078
发表时间：
1992-11
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
R. Yolken;J. Peterson;S. Vonderfecht;E. Fouts;K. Midthun;D. Newburg
通讯作者：
R. Yolken;J. Peterson;S. Vonderfecht;E. Fouts;K. Midthun;D. Newburg
影响因子：
5.6
作者：
PRASAD, BVV;WANG, GJ;CHIU, W
通讯作者：
CHIU, W
胶原蛋白中α2β1整合素四肽识别序列的鉴定。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Staatz,WD;Fok,KF;Zutter,MM;Adams,SP;Rodriguez,BA;Santoro,SA
通讯作者：
Santoro,SA