Evaluation of a broadly protective Chlamydia-cholera combination vaccine candidate.
Evaluation of a broadly protective Chlamydia-cholera combination vaccine candidate.
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DOI：
10.1016/j.vaccine.2011.03.027
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发表时间：
2011-05-12
期刊：
影响因子：
5.5
通讯作者：
Igietseme, J. U.
中科院分区：
文献类型：
--
作者：
Eko, F. O.;Okenu, D. N.;Singh, U. P.;He, Q.;Black, C.;Igietseme, J. U.
需要用单一疫苗同时靶向人群中流行病学重叠的感染，这为开发联合疫苗提供了基础。霍乱弧菌菌影（rVCG）为开发针对许多人类和动物病原体的疫苗提供了一种有吸引力的方法。在这项研究中，我们构建了一个多亚单位疫苗候选人共表达血清型D衍生孔蛋白B和多态性膜蛋白D蛋白的沙眼衣原体，并评估其能力，同时诱导广泛的衣原体免疫，并引发杀弧菌抗体反应的弧菌载体包膜。候选疫苗的肌内（IM）免疫可诱导针对异源血清型和菌株（包括血清型E、F、G、H和L）的高水平抗原特异性生殖器粘膜和全身性Th 1细胞介导和体液免疫应答。此外，除了多亚单位疫苗外，单亚单位构建体对异源小鼠品系C. muridarum。此外，所有小鼠免疫rVCG疫苗结构的反应与杀弧菌抗体滴度，在cholera保护的替代标记显着上升。这些研究结果表明，多亚单位疫苗诱导保护性cross-protectivechlamydial以及asvibriocidal immunityand建立一个广泛有效的衣原体-霍乱联合疫苗的可能性。
The need to simultaneously target infections with epidemiological overlap in the population with a single vaccine provides the basis for developing combination vaccines. Vibrio cholerae ghosts (rVCG) offer an attractive approach for developing vaccines against a number of human and animal pathogens. In this study, we constructed a multisubunit vaccine candidate co-expressing the serovar D-derived Porin B and polymorphic membrane protein-D proteins of Chlamydia trachomatis and evaluated its ability to simultaneously induce broad-based chlamydial immunity and elicit a vibriocidal antibody response to the Vibrio carrier envelope. Intramuscular (IM) immunization with the vaccine candidate elicited high levels of antigen-specific genital mucosal and systemic Th1 cell-mediated and humoral immune responses against heterologous serovars and strains, including serovars E, F, G, H and L. Also, in addition to the multisubunit vaccine, the single subunit constructs conferred significant cross protection against the heterologous mouse strain, C. muridarum. Furthermore, all mice immunized with rVCG vaccine constructs responded with a significant rise in vibriocidal antibody titer, the surrogate marker for protection in cholera.These findings demonstrate the ability of the multisubunit vaccine to inducecross protectivechlamydial as well asvibriocidal immunityand establish the possibility of developing a broadly efficacious Chlamydia-cholera combination vaccine.
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影响因子：
4.4
作者：
Eko, FO;He, Q;Igietseme, JU
通讯作者：
Igietseme, JU
影响因子：
4.8
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4.4
作者：
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通讯作者：
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5.5
作者：
Eko, F. O.;Ekong, E.;Igietseme, J. U.
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Igietseme, J. U.
影响因子：
5.5
作者：
Eko, FO;Schukovskaya, T;Lubitz, W
通讯作者：
Lubitz, W