Cell Placement and Neural Guidance Using a Three-Dimensional Microfluidic Array
Cell Placement and Neural Guidance Using a Three-Dimensional Microfluidic Array
复制标题
使用三维微流体阵列进行细胞放置和神经引导
DOI：
10.1143/jjap.40.5485
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发表时间：
2001
影响因子：
1.5
通讯作者：
H. Fujita
中科院分区：
文献类型：
--
作者：
L. Griscom;P. Degenaar;B. Lepioufle;E. Tamiya;H. Fujita
介绍了用于生物细胞构图和单神经元引导的微孔三维阵列的几种制作技术。探索了在聚二甲基硅氧烷(PDMS)膜中制作复杂的3D高深宽比结构的方法。本工作采用电感耦合等离子体反应离子刻蚀技术，利用SU-8负性光刻胶，直接在硅片上制作三维微模。单元放置是通过放置在玻璃基板上的150-100微米厚的PDMS膜上的50微米方孔阵列来实现的。膜上的垂直孔由膜玻璃一侧的水平隧道连接，用于神经指导或药物或营养物质的输送。使用荧光酵母细胞和PC12神经细胞测试了该膜对细胞放置、生长和引导的有效性。
Several fabrication techniques for making three-dimensional arrays of micro-wells for biological cell patterning and single-neuron guidance are presented. Methods for making complex 3d high-aspect-ratio structures in poly-dimethylsiloxane (PDMS) membrane are explored. In this work, three-dimensional micro-molds are made directly on silicon wafers though inductively coupled plasma reactive ion etching (ICP-RIE), and also using SU-8 negative photoresist. Cell placement is achieved through an array of 50-µm square holes in a 150–100 µm thick PDMS membrane, which is placed on a glass substrate. Vertical holes in the membrane are linked by horizontal tunnels on the glass side of the membrane, for use in neural guidance or delivery of drugs or nutrients. The effectiveness of the membrane for cell placement, growth and guidance was tested using fluorescent yeast cells and PC12 neuronal cells.
影响因子：
7.4
作者：
Anderson, JR;Chiu, DT;Whitesides, GM
通讯作者：
Whitesides, GM