Reduction of anisotropic image resolution in transmission electron microtomography by use of quadrangular prism-shaped section.
Reduction of anisotropic image resolution in transmission electron microtomography by use of quadrangular prism-shaped section.
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通过使用四棱柱形截面降低透射电子显微断层扫描中的各向异性图像分辨率。
DOI：
10.1093/jmicro/dfi065
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
H. Jinnai
中科院分区：
文献类型：
--
作者：
T. Kaneko;H. Nishioka;T. Nishi;H. Jinnai
在尽可能宽的倾斜范围内，通过透射电子显微断层扫描（TEMT）在三维（3-D）中观察四边形棱镜标本（“棱镜形”切片）。制备了两种类型的样品用于TEMT实验：(i)嵌段共聚物纳米结构的棱柱形截面，其横截面每侧为200nm； （ii）具有与棱柱形截面相同厚度（约200nm）的常规超薄截面。棱柱形切片在高倾斜角度（如60度）下的投影图像质量明显优于超薄切片。这是因为在相同的倾斜角度下，棱镜形截面的电子束路径长度比超薄截面的电子束路径长度短（因此透射率更高）。因此，虽然超薄切片的倾斜角度大于约40度时的投影不能有效地进行三维重建，但棱柱形切片的倾斜角度非常大，如+/-75度，仍然可以用于三维重建。对三维重建的三个正交截面图进行了广泛的比较。结果发现：(1)三维重建的图像对比度明显增强；(2)由于电子断层扫描角度范围的限制，结构物体的伸长率明显降低。
A quadrangular prism specimen (a 'prism-shaped' section) was observed in three-dimension (3-D) by transmission electron microtomography (TEMT) over as wide a tilt range as possible. Two types of specimens were prepared for the TEMT experiments: (i) a prism-shaped section of a block copolymer nanostructure, whose cross section was 200 nm on each side and (ii) a conventional ultrathin section having the same thickness (approximately 200 nm) as the prism-shaped section. Image quality of the projections taken at high tilt angles, e.g. 60 degrees, of the prism-shaped section was considerably better than that of the ultrathin section. This was because the path length of electron beam of the prism-shaped section was shorter (and hence the transmission was higher) than that of the ultrathin section at the same tilt angle. Thus, although the projections of the ultrathin section at the tilt angle larger than approximately 40 degrees did not effectively contribute to the 3-D reconstruction, those of the prism-shaped section at very high tilt angles, e.g. +/-75 degrees, can still be used for the 3-D reconstruction. Three orthogonal cross-sectional views of the 3-D reconstruction were extensively compared between the two sections. It was found that (i) the image contrast of the 3-D reconstruction was significantly enhanced and (ii) an elongation of structural object due to limitation of the angular range in the electron tomography was considerably reduced using the prism-shaped section.
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影响因子：
2.2
作者：
PENCZEK, P;MARKO, M;FRANK, J
通讯作者：
FRANK, J
影响因子：
3
作者：
Mastronarde, DN
通讯作者：
Mastronarde, DN
直接测量双连续嵌段共聚物形态中的界面曲率分布。
DOI：
10.1103/physrevlett.84.518
发表时间：
2000
期刊：
Physical review letters.
影响因子：
--
作者：
Jinnai,H;Nishikawa,Y;Spontak,RJ;Smith,SD;Agard,DA;Hashimoto,T
通讯作者：
Hashimoto,T
透射电子显微镜
DOI：
10.1007/978-3-319-26651-0_3
发表时间：
2016
期刊：
--
影响因子：
--
作者：
Burke M
通讯作者：
Burke M