Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors.
Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors.
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DOI：
10.1186/1475-2875-11-374
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发表时间：
2012-11-14
期刊：
影响因子：
3
通讯作者：
Fillinger U
中科院分区：
文献类型：
--
作者：
Dugassa S;Lindh JM;Torr SJ;Oyieke F;Lindsay SW;Fillinger U
人们对疟蚊在自然界中如何定位产卵地点知之甚少。这些知识对于帮助制定可用于针对怀孕女性的监测和控制措施非常重要。本研究旨在开发一套工具，用于研究妊娠冈比亚按蚊的吸引力。与水生栖息地相关的视觉或嗅觉线索。首先，该研究开发和评估了使用电击网来分析怀孕女性对水生栖息地的取向的方法。电网（1 m高× 0.5m宽）由12 V电池通过火花盒供电。高和低能量设置进行了比较，蚊子触电和收集设备的发展，以保持触电蚊子时，落在地上。其次，测试了一系列粘性材料和洗涤剂，通过处理池塘边缘和水面来量化怀孕的雌性是否以及在哪里着陆产卵。所有实验均采用随机完全区组设计，每天释放200只蚊子。试验在半田间筛选系统中进行。冈比亚公司数据用广义估计方程进行分析。一个电网在400伏直流电的最高火花盒能量下运行，使电网产生火花，产生噼啪声，一阵亮光和烧焦的气味。这种设置比由既听不见也看不见的降低电压输出供电的蚊帐捕获的蚊子少64%（比值比（OR）0.46; 95%置信区间（CI）0.40-0.53，p < 0.001）。三块粘板对透明膜、涂有胶水的黑色捕蝇网和黄色膜作为电网下的捕蝇装置进行了评价，发现透明和有光泽的黑色表面具有高度吸引力001和OR 28.8，95%CI 14.5 - 56.8，p < 0.001），因此不适合作为电子蚊帐下的收集装置。用围绕池塘的四个电子网的正方形与黄色粘板组合，平均收集33%（95%CI 28-38%）的释放的蚊子。当蚊子落在池塘边或水面上时，水中的粘性材料和洗涤剂对收集蚊子很有效。与人工池塘边缘（平均值24，95% CI 20-28）相比，在水面（平均值103，95% CI 93-115）上发现了超过80%的采集雌性。一个正方形的四个电子网与黄色粘板作为收集装置，可用于量化接近一个小产卵地点的蚊子数量。有光泽的粘性表面吸引怀孕的女性可能是因为他们在视觉上被误认为是水生栖息地。这些材料可进一步发展为重力圈闭。冈比亚按蚊主要在水面上产卵。这种行为可用于开发新的诱捕和控制策略。
Little is known about how malaria mosquitoes locate oviposition sites in nature. Such knowledge is important to help devise monitoring and control measures that could be used to target gravid females. This study set out to develop a suite of tools that can be used to study the attraction of gravid Anopheles gambiae s.s. towards visual or olfactory cues associated with aquatic habitats. Firstly, the study developed and assessed methods for using electrocuting nets to analyse the orientation of gravid females towards an aquatic habitat. Electric nets (1m high × 0.5m wide) were powered by a 12V battery via a spark box. High and low energy settings were compared for mosquito electrocution and a collection device developed to retain electrocuted mosquitoes when falling to the ground. Secondly, a range of sticky materials and a detergent were tested to quantify if and where gravid females land to lay their eggs, by treating the edge of the ponds and the water surface. A randomized complete block design was used for all experiments with 200 mosquitoes released each day. Experiments were conducted in screened semi-field systems using insectary-reared An. gambiae s.s. Data were analysed by generalized estimating equations. An electric net operated at the highest spark box energy of a 400 volt direct current made the net spark, creating a crackling sound, a burst of light and a burning smell. This setting caught 64% less mosquitoes than a net powered by reduced voltage output that could neither be heard nor seen (odds ratio (OR) 0.46; 95% confidence interval (CI) 0.40-0.53, p < 0.001). Three sticky boards (transparent film, glue coated black fly-screen and yellow film) were evaluated as catching devices under electric nets and the transparent and shiny black surfaces were found highly attractive (OR 41.6, 95% CI 19.8 – 87.3, p < 0.001 and OR 28.8, 95% CI 14.5 – 56.8, p < 0.001, respectively) for gravid mosquitoes to land on compared to a yellow sticky film board and therefore unsuitable as collection device under the e-nets. With a square of four e-nets around a pond combined with yellow sticky boards on average 33% (95% CI 28-38%) of mosquitoes released were collected. Sticky materials and detergent in the water worked well in collecting mosquitoes when landing on the edge of the pond or on the water surface. Over 80% of collected females were found on the water surface (mean 103, 95% CI 93–115) as compared to the edge of the artificial pond (mean 24, 95% CI 20–28). A square of four e-nets with yellow sticky boards as a collection device can be used for quantifying the numbers of mosquitoes approaching a small oviposition site. Shiny sticky surfaces attract gravid females possibly because they are visually mistaken as aquatic habitats. These materials might be developed further as gravid traps. Anopheles gambiae s.s. primarily land on the water surface for oviposition. This behaviour can be exploited for the development of new trapping and control strategies.
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影响因子：
1.9
作者：
Facchinelli, L.;Valerio, L.;Della Torre, A.
通讯作者：
Della Torre, A.
DOI：
10.4269/ajtmh.2008.78.904
发表时间：
2008-06-01
影响因子：
3.3
作者：
Facchinelli, Luca;Koenraadt, Constantianus J. M.;della Torre, Alessandra
通讯作者：
della Torre, Alessandra
影响因子：
3.2
作者：
Harris, Caroline;Kihonda, Japhet;Majambere, Silas
通讯作者：
Majambere, Silas
影响因子：
1.9
作者：
Gaugler, R.;Suman, D.;Wang, Y.
通讯作者：
Wang, Y.
影响因子：
1.7
作者：
Braks, Marieta A. H.;Carde, Ring T.
通讯作者：
Carde, Ring T.