Novel protein-protein contacts facilitate mRNA 3'-processing signal recognition by Rna15 and Hrp1.
Novel protein-protein contacts facilitate mRNA 3'-processing signal recognition by Rna15 and Hrp1.
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DOI：
10.1016/j.jmb.2010.06.032
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发表时间：
2010-08-20
影响因子：
5.6
通讯作者：
Varani G
中科院分区：
文献类型：
--
作者：
Leeper TC;Qu X;Lu C;Moore C;Varani G
精确的3‘端处理对于正确的基因表达是必不可少的，然而在酵母中，3’端处理信号由多个模糊的序列元件组成。裂解位点上游的两个相邻元件对于3‘-加工的准确性(定位元件)和效率(效率元件)特别重要，分别被RNA结合蛋白Rna 15和Hrp1识别。在体内，这些相互作用被稳定它们联系的支架蛋白Rna 14加强。通过剩余偶极耦合和顺磁驰豫实验确定了Hrp1和Rna 15的RRM结构域与这对RNA元件结合的34 kDa三元络合物的核磁共振结构。它揭示了每种蛋白质是如何与RNA结合的，并在以前未知的功能区域引入了一类新的蛋白质-蛋白质接触。这些域间接触以前在其他多RRM结构中被忽视，尽管仔细分析表明它们可能经常存在。这些接触区域的突变破坏了3‘-端的加工，表明它们可能在结构上组织了负责RNA加工的核糖核蛋白(RNP)复合体。
Precise 3′-end processing of mRNA is essential for correct gene expression, yet in yeast, 3′- processing signals consist of multiple ambiguous sequence elements. Two neighboring elements upstream of the cleavage site are particularly important for the accuracy (positioning element) and efficiency (efficiency element) of 3′-processing and are recognized by the RNA-binding proteins Rna15 and Hrp1, respectively. In vivo, these interactions are strengthened by the scaffolding protein Rna14 that stabilizes their association. The NMR structure of the 34 kDa ternary complex of the RRM domains of Hrp1 and Rna15 bound to this pair of RNA elements was determined using Residual Dipolar Coupling and Paramagnetic relaxation experiments. It reveals how each of the proteins binds to RNA, and introduces a novel class of protein-protein contact in regions of previously unknown function. These interdomain contacts had previously been overlooked in other multi-RRM structures, although a careful analysis suggests that they may be frequently present. Mutations in the regions of these contacts disrupt 3′-end processing, suggesting that they may structurally organize the ribonucleoprotein (RNP) complexes responsible for RNA processing.
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影响因子：
4.8
作者：
Enokizono, Y;Konishi, Y;Katahira, M
通讯作者：
Katahira, M
影响因子：
2.2
作者：
Clore, GM;Gronenborn, AM;Tjandra, N
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影响因子：
11.4
作者：
Allain, FHT;Howe, PWA;Varani, G
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影响因子：
14.9
作者：
Graber, JH;Cantor, CR;Smith, TF
通讯作者：
Smith, TF
DOI：
10.1073/pnas.101046598
发表时间：
2001-05-22
影响因子：
11.1
作者：
Gross, S;Moore, C
通讯作者：
Moore, C