Establishment of a primary human sarcoma model in athymic nude mice
Establishment of a primary human sarcoma model in athymic nude mice
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无胸腺裸鼠原代人肉瘤模型的建立
DOI：
10.1111/j.1749-0774.2010.00085.x
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发表时间：
--
期刊：
影响因子：
4.3
通讯作者：
Al-Benna S
中科院分区：
文献类型：
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作者：
Steinstraesser L;Jacobsen F;Schubert C;Gevers K;Steinau HU;Al-Benna S
改善软组织肉瘤患者的预后需要良好的动物模型，以评估新的治疗选择。异种移植肉瘤模型经常使用，但通常使用已建立的细胞系，而不是原代人肉瘤细胞。本研究的目的是建立一种可重复的人原发性软组织肉瘤裸鼠移植模型。从四个切除的人肉瘤中分离原代软组织肉瘤细胞，培养至第三代，并注射到裸鼠皮下。通过组织学和免疫组织化学染色进一步分析肉瘤异种移植物。在四分之二的肉瘤中，可以成功地建立肿瘤生长，导致高达540 mm3体积的实体瘤。组织学和免疫组织化学染色证实小鼠异种移植物与原始患者的组织相比是相同的肉瘤。本研究建立了人原发性软组织肉瘤裸鼠移植瘤模型。该动物模型对研究肉瘤的发生和治疗具有重要意义。
Improvement of soft tissue sarcoma patient outcome requires well-characterized animal models in which to evaluate novel therapeutic options. Xenograft sarcoma models are frequently used, but commonly with established cell lines rather than with primary human sarcoma cells. The objective of the present study was to establish a reproducible xenograft model of primary human soft tissue sarcoma in athymic nude mice. Primary soft tissue sarcoma cells from four resected human sarcomas were isolated, cultured until the third passage and injected subcutaneously into athymic nude mice. The sarcoma xenograft was further analyzed by histological and immunohistochemical staining. In two out of four sarcomas tumor growth could successfully be established leading to solid tumors of up to 540 mm3volume. Histological and immunohistochemical staining confirmed the mouse xenograft as identical sarcoma compared with the original patient’s tissue. In the present study a reproducible xenograft model of primary human soft tissue sarcoma in athymic nude mice was established. This animal model is of great interest for the study of sarcomogenesis and therapy.
人类软组织和骨肉瘤治疗的分子靶点
影响因子：
4.7
作者：
D. Steinert;L. Blakely;J. Salganick;J. Trent
通讯作者：
J. Trent
软组织肉瘤的转移模式。
影响因子：
--
作者：
M. Vezeridis;R. Moore;C. Karakousis
通讯作者：
C. Karakousis
世界卫生组织肿瘤分类：软组织和骨肿瘤的病理学和遗传学：C. D. M. Fletcher K. K. Unni 和 F. Mertens 编辑，第 427 页，里昂，法国：IARC Press，2002 年，ISBN：9-2832-2413-2 , £53.00
DOI：
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发表时间：
2004
期刊：
影响因子：
--
作者：
D. Mangham
通讯作者：
D. Mangham