Localization, with Monoclonal Antibodies and by Detection of Autonomous Fluorescence, of Blood Cells in the Tissues of the Vanadium-Rich Ascidian, Ascidia sydneiensis samea

Localization, with Monoclonal Antibodies and by Detection of Autonomous Fluorescence, of Blood Cells in the Tissues of the Vanadium-Rich Ascidian, Ascidia sydneiensis samea
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通过单克隆抗体和自主荧光检测,对富含钒的海鞘(Ascidia sydneiensis Samea)组织中的血细胞进行定位

DOI:
10.2108/zsj.12.733
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发表时间:
1995
影响因子:
3.9
通讯作者:
H. Michibata
H. Michibata
中科院分区:
生物学3区
文献类型:
--
作者:
A. Kaneko;T. Uyama;Y. Moriyama;H. Michibata

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海鞘有几种类型的血细胞,据报道这些血细胞参与各种功能。然而,目前还不清楚哪种类型的血细胞参与了腹水中的每一种功能,以及每种类型的血细胞位于腹水中的哪个组织。作为弄清这些重要和棘手问题的必要步骤,我们用两种细胞标记物--单抗和自主荧光检测了四种不同类型血细胞的定位。结果表明,印戒细胞是唯一分布于消化道周围结缔组织中的血细胞,而变形细胞主要分布于鳃篮横血管中。桑椹细胞和室细胞分布于内脏区域周围的外套膜下。
Abstract Ascidians have several types of blood cells which have been reported to be involved in various functions. However, it is still unclear what type of blood cell participates in each of various functions and in which tissue each type of blood cell localize in ascidians. As a necessary step to make clear the important and intractable problems, the localization of four different types of blood cells was examined using two kinds of cell marker, monoclonal antibodies and autonomous fluorescence. Consequently, it was revealed that signet ring cells were the only blood cells that were localized in the connective tissues around the alimentary canal and the amoebocytes were mainly distributed in the transverse vessels of the branchial basket. The morula cells and compartment cells were distributed under the epidermis of the mantle around the visceral region.
道端仁:实验动物学杂志。
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DOI: 10.1002/jez.1402570304
发表时间: 1991-03-01
影响因子: --
作者:
MICHIBATA, H;IWATA, Y;HIRATA, J
通讯作者: HIRATA, J