Estrogen provision by reactive glia decreases apoptosis in the zebra finch (Taeniopygia guttata).
Estrogen provision by reactive glia decreases apoptosis in the zebra finch (Taeniopygia guttata).
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反应性神经胶质细胞提供的雌激素可减少斑胸草雀（Taeniopygia guttata）的细胞凋亡。
DOI：
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Ryan D. Wynne
中科院分区：
文献类型：
--
作者：
C. Saldanha;Kevin N Rohmann;Luckshman Coomaralingam;Ryan D. Wynne
神经损伤部位神经胶质细胞中芳香化酶(雌激素合成酶)的上调可能会限制神经退变。全身应用雌激素限制了神经损伤，但局部雌激素供应在这一作用中的具体作用尚不清楚。在雄性斑马雀身上，我们测试了局部芳香酶抑制和雌激素替代对细胞退化类型的影响，以及这种退化距离侮辱来源的距离。受试者接受一侧端脑叶注射芳香酶抑制剂Fadrozole，对侧脑叶注射Fadrozole和雌二醇。72小时后，我们用荧光素B和TUNEL分别标记死亡细胞和凋亡细胞。由于每个受试者都有自己的对照，我们能够相对区别地评估局部雌激素替代与循环类固醇和结构性芳构化的影响。用荧光素B、原位末端标记法和芳香化酶表达间接检测病变周围的细胞变性。此外，损伤周围芳香酶阳性细胞的胶质性质通过与波形蛋白的共同定位而被质疑。与单独注射Fadrozole的大脑半球相比，雌激素替代的损伤有更少的凋亡细胞聚集在损伤周围。由于Fluoro-Jade B和TUNEL标记的细胞数量相似，而且这些细胞到注射的距离相同，我们认为在当前的范例中，雌激素替代主要起到限制细胞凋亡的作用。最后，损伤周围的芳香酶阳性细胞与波形蛋白共定位，确立了它们的神经胶质性质。因此，在神经损伤部位提供神经胶质雌激素可能是通过抑制细胞凋亡来限制损伤引起的细胞退化的关键。
Upregulation of aromatase (estrogen synthase) in glia around the site of neural injury may limit neural degeneration. Systemic administration of estrogen limits neural damage, but the specific role of local estrogen provision in this effect is unclear. In male zebra finches, we tested the effect of local aromatase inhibition and estrogen replacement on type of cellular degeneration and the distance of this degeneration from the source of insult. Subjects received injections of the aromatase inhibitor fadrozole into one telencephalic lobe and fadrozole and estradiol into the contralateral lobe. Seventy-two hours later, we used Fluoro-Jade B and TUNEL to label dying and apoptotic cells, respectively. Since each subject was its own control, we were able to assess the influence of local estrogen replacement in relative distinction from circulating steroids and constitutive aromatization. Cellular degeneration around the lesion was measured with Fluoro-Jade B, TUNEL, and indirectly with aromatase expression. Additionally, the glial nature of aromatase-positive cells around the injury was queried by co-localization with vimentin. The estrogen replaced injury had fewer apoptotic cells clustered more closely around the injury compared to the hemisphere injected with fadrozole alone. Since Fluoro-Jade B and TUNEL labeled similar numbers of cells, and the distance of these cells from the injection was identical, we suggest that estrogen replacement functions primarily to restrict apoptosis in the current paradigm. Lastly, aromatase-positive cells around injuries co-localize vimentin, establishing their glial nature. Thus, glial estrogen provision at sites of neural insult may be critical in limiting the cellular degeneration caused by injury via an inhibition of apoptosis.
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影响因子：
8.3
作者：
Rusa, R;Alkayed, NJ;Hurn, PD
通讯作者：
Hurn, PD
雄性鸣禽体内循环的雌激素源自大脑。
DOI：
10.1073/pnas.89.16.7650
发表时间：
1992
影响因子：
11.1
作者：
Schlinger,BA;Arnold,AP
通讯作者：
Arnold,AP
Fadrozole：斑胸草雀大脑中芳香酶的一种有效且特异性的抑制剂。
影响因子：
2.7
作者：
Wade,J;Schlinger,BA;Hodges,L;Arnold,AP
通讯作者：
Arnold,AP
雌激素的组织保护作用涉及半胱天冬酶基因表达的调节。
DOI：
10.1210/mend.16.6.0855
发表时间：
2002
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Monroe,DavidG;Berger,RyanR;Sanders,MichelM
通讯作者：
Sanders,MichelM
DOI：
10.1073/pnas.88.10.4191
发表时间：
1991-05-01
影响因子：
11.1
作者：
SCHLINGER, BA;ARNOLD, AP
通讯作者：
ARNOLD, AP