A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos.
A hierarchy of H3K4me3 and H3K27me3 acquisition in spatial gene regulation in Xenopus embryos.
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DOI：
10.1016/j.devcel.2009.08.005
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发表时间：
2009-09
影响因子：
11.8
通讯作者：
Veenstra, Gert Jan C.
中科院分区：
文献类型：
--
作者：
Akkers, Robert C.;van Heeringen, Simon J.;Jacobi, Ulrike G.;Janssen-Megens, Eva M.;Francoijs, Kees-Jan;Stunnenberg, Hendrik G.;Veenstra, Gert Jan C.
表观遗传机制将多细胞生物基因组中的活性和非活性区域分开，以在胚胎发生期间产生不同的细胞命运。在这里，我们报告的表观遗传和转录组基因组全基因组地图原肠胚阶段非洲爪蟾胚胎使用大规模平行测序的cDNA（RNA-seq）和DNA获得的染色质免疫沉淀（ChIP-seq）的组蛋白H3 K4和K27三甲基化和RNA聚合酶II（RNAPII）。这些图谱鉴定启动子和转录区域。引人注目的是，具有相反的组蛋白修饰的基因组区域主要被转录，反映了空间调控的表达，而不是二价，如通过表达谱分析、连续ChIP和ChIP-seq对解剖胚胎所确定的。H3 K27 me 3沉积的空间差异是局部基因表达的预测。此外，H3 K4 me 3的出现与合子基因的激活相一致，而H3 K27 me 3主要沉积在随后的空间限制或抑制转录调节因子。这些结果揭示了合子基因激活的空间控制层次。
Epigenetic mechanisms set apart the active and inactive regions in the genome of multicellular organisms to produce distinct cell fates during embryogenesis. Here we report on the epigenetic and transcriptome genome-wide maps of gastrula-stage Xenopus tropicalis embryos using massive parallel sequencing of cDNA (RNA-seq) and DNA obtained by chromatin immunoprecipitation (ChIP-seq) of histone H3 K4 and K27 trimethylation and RNA Polymerase II (RNAPII). These maps identify promoters and transcribed regions. Strikingly, genomic regions featuring opposing histone modifications are mostly transcribed, reflecting spatially regulated expression rather than bivalency as determined by expression profile analyses, sequential ChIP, and ChIP-seq on dissected embryos. Spatial differences in H3K27me3 deposition are predictive of localized gene expression. Moreover, the appearance of H3K4me3 coincides with zygotic gene activation, whereas H3K27me3 is predominantly deposited upon subsequent spatial restriction or repression of transcriptional regulators. These results reveal a hierarchy in the spatial control of zygotic gene activation.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
64.5
作者：
Guenther, Matthew G.;Levine, Stuart S.;Young, Richard A.
通讯作者：
Young, Richard A.
影响因子：
2.7
作者：
Engleka, MJ;Craig, EJ;Kessler, DS
通讯作者：
Kessler, DS
影响因子：
14.9
作者：
Bowes JB;Snyder KA;Segerdell E;Gibb R;Jarabek C;Noumen E;Pollet N;Vize PD
通讯作者：
Vize PD
影响因子：
23.9
作者：
Cui K;Zang C;Roh TY;Schones DE;Childs RW;Peng W;Zhao K
通讯作者：
Zhao K