Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.
Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.
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DOI：
10.1093/nar/gkac605
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发表时间：
2022-08-12
影响因子：
14.9
通讯作者：
Gebauer, Fatima
中科院分区：
文献类型：
--
作者：
Mestre-Farras, Neus;Guerrero, Santiago;Bley, Nadine;Rivero, Ezequiel;Coll, Olga;Borras, Eva;Sabido, Eduard;Indacochea, Alberto;Casillas-Serra, Carlos;Jarvelin, Aino, I;Oliva, Baldomero;Castello, Alfredo;Huettelmaier, Stefan;Gebauer, Fatima
RNA-binding proteins (RBPs) have been relatively overlooked in cancer research despite their contribution to virtually every cancer hallmark. Here, we use RNA interactome capture (RIC) to characterize the melanoma RBPome and uncover novel RBPs involved in melanoma progression. Comparison of RIC profiles of a non-tumoral versus a metastatic cell line revealed prevalent changes in RNA-binding capacities that were not associated with changes in RBP levels. Extensive functional validation of a selected group of 24 RBPs using five different in vitro assays unveiled unanticipated roles of RBPs in melanoma malignancy. As proof-of-principle we focused on PDIA6, an ER-lumen chaperone that displayed a novel RNA-binding activity. We show that PDIA6 is involved in metastatic progression, map its RNA-binding domain, and find that RNA binding is required for PDIA6 tumorigenic properties. These results exemplify how RIC technologies can be harnessed to uncover novel vulnerabilities of cancer cells.
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影响因子：
7.3
作者：
Gao J;Aksoy BA;Dogrusoz U;Dresdner G;Gross B;Sumer SO;Sun Y;Jacobsen A;Sinha R;Larsson E;Cerami E;Sander C;Schultz N
通讯作者：
Schultz N
影响因子：
8
作者：
Damsky WE;Bosenberg M
通讯作者：
Bosenberg M
DOI：
10.1016/j.tem.2015.09.012
发表时间：
2015-12
期刊：
Trends in endocrinology and metabolism: TEM
影响因子：
--
作者：
Castello A;Hentze MW;Preiss T
通讯作者：
Preiss T
影响因子：
14.8
作者：
Castello, Alfredo;Horos, Rastislav;Hentze, Matthias W.
通讯作者：
Hentze, Matthias W.
影响因子：
5.8
作者：
Esmaillie, Reza;Ignarski, Michael;Fabretti, Francesca
通讯作者：
Fabretti, Francesca