Detection of muscle stem cell-derived myonuclei in murine overloaded muscles.
Detection of muscle stem cell-derived myonuclei in murine overloaded muscles.
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超负荷小鼠肌肉中肌干细胞源性肌细胞核的检测。
DOI：
10.1016/j.xpro.2022.101307
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发表时间：
2022-06-17
期刊：
影响因子：
--
通讯作者：
Fukada, So-ichiro
中科院分区：
文献类型：
--
作者：
Kaneshige, Akihiro;Kaji, Takayuki;Saito, Hayato;Higashimoto, Tatsuyoshi;Nakamura, Ayasa;Kurosawa, Tamaki;Ikemoto-Uezumi, Madoka;Uezumi, Akiyoshi;Fukada, So-ichiro
肌卫星细胞（Muscle satellite cells，MuSC）为现有的肌纤维提供细胞核以响应机械负荷。这种肌肉增生对于有效的肌肉肥大至关重要。在本文中，我们提出了用于在负载的小鼠肌肉中检测MuSC衍生的新肌核的方案，包括用于EdU注射以染色肌核，然后进行手术和骨骼肌固定的程序。然后，我们描述了免疫染色EdU+肌细胞核和图像采集定量分析。有关本方案使用和执行的完整详细信息，请参阅。跖肌机械负荷的外科手术制备冷冻肌肉样品的基本程序EdU+肌核免疫染色的详细描述获取图像进行定量分析肌肉卫星细胞（MuSC）响应机械负荷向现有肌纤维提供核。这种肌肉增生对于有效的肌肉肥大至关重要。在本文中，我们提出了用于在负载的小鼠肌肉中检测MuSC衍生的新肌核的方案，包括用于EdU注射以染色肌核，然后进行手术和骨骼肌固定的程序。然后，我们描述了免疫染色EdU+肌细胞核和图像采集定量分析。
Muscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for EdU injection to stain myonuclei, followed by surgery and skeletal muscle fixation. We then describe immunostaining for EdU+ myonuclei and image acquisition for quantitative analyses. For complete details on the use and execution of this protocol, please refer to. Surgical procedure for mechanical loading of the plantaris muscle Fundamental procedure for preparing frozen muscle samples Detailed description of immunostaining for EdU+ myonuclei Acquisition of images for quantitative analyses Muscle satellite cells (MuSCs) supply nuclei to existing myofibers in response to mechanical loading. This myonuclear accretion is critical for efficient muscle hypertrophy. Herein, we present protocols for the detection of MuSC-derived new myonuclei in loaded mouse muscle, including procedures for EdU injection to stain myonuclei, followed by surgery and skeletal muscle fixation. We then describe immunostaining for EdU+ myonuclei and image acquisition for quantitative analyses.
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影响因子：
4.6
作者：
McCarthy, John J.;Mula, Jyothi;Peterson, Charlotte A.
通讯作者：
Peterson, Charlotte A.
影响因子：
4.8
作者：
Fry, Christopher S.;Lee, Jonah D.;Peterson, Charlotte A.
通讯作者：
Peterson, Charlotte A.
影响因子：
4.9
作者：
Murach KA;White SH;Wen Y;Ho A;Dupont-Versteegden EE;McCarthy JJ;Peterson CA
通讯作者：
Peterson CA
影响因子：
7.7
作者：
Fukuda, Sumiaki;Kaneshige, Akihiro;Fukada, So-ichiro
通讯作者：
Fukada, So-ichiro
影响因子：
64.8
作者：
Lepper C;Conway SJ;Fan CM
通讯作者：
Fan CM