Effects of Coumestrol and Equol on the Developing Reproductive Tract of the Rat
Effects of Coumestrol and Equol on the Developing Reproductive Tract of the Rat
复制标题
香雌酚和雌马酚对大鼠生殖道发育的影响
DOI：
10.3181/00379727-208-43833
复制
发表时间：
1995
影响因子：
--
通讯作者：
D. Sheehan
中科院分区：
文献类型：
--
作者：
K. Medlock;W. Branham;D. Sheehan
摘要植物雌激素香豆雌酚和雌马酚是弱雌激素。在这里，我们已经研究了它们在新生大鼠子宫中诱导反应的能力。强效雌激素如己烯雌酚（DES）和17β-雌二醇在出生后第1-5天给药时，最初使出生后第5天（PND）的子宫重量增加一倍，随后在以后的年龄降低子宫生长和腺体发育。在本研究中，Sprague-Dawley幼仔经阴道（PND 1-5）给予不同剂量的香豆雌酚和雌马酚，并在不同年龄处死，以确定生化和形态学终点的变化。在腺体发生的关键时期（PND 10-14），向其他大鼠注射相同的化合物，以检查其对腺体发育的影响。在PND 1-5，100 μg香豆雌酚剂量下，观察到腺体过早发育和子宫重量增加。然而，在以后的年龄，子宫重量显着降低，有一个严重的抑制雌激素受体（ER）水平。雌马酚降低子宫重量在以后的年龄，但不影响ER水平。当在PND 10-14给药时，香豆雌酚和雌马酚均引起腺体发生的剂量依赖性抑制，尽管不如DES或他莫昔芬严重。香豆雌酚作为雌激素的效力比雌马酚高约103倍，其对子宫重量、腺体和ER水平的影响与DES相似。在本研究中使用的剂量下，雌马酚未能表现出雌激素或抗雌激素活性。
Abstract The phytoestrogens, coumestrol and equol, are weakly estrogenic. Here, we have examined their ability to induce responses in the neonatal rat uterus. Potent estrogens such as diethylstilbestrol (DES) and 17β-estradiol which initially double uterine weight on postnatal Day (PND) 5 when given on PND 1-5 subsequently reduce both uterine growth and gland development at later ages. In this study, Sprague-Dawley pups were treated neonatally (PND 1-5) with various doses of coumestrol and equol, and sacrificed at different ages to determine alterations in biochemical and morphological endpoints. Other rats were injected with the same compounds during the critical period of gland genesis (PND 10-14) to examine their effects on gland development. At the 100 μg coumestrol dose, on PND 1-5, premature gland development and increased uterine weight were observed. However, at later ages, uterine weight was significantly lowered and there was a severe suppression in the estrogen receptor (ER) levels. Equol lowered uterine weight at the later ages but did not affect ER levels. When given on PND 10-14, both coumestrol and equol caused a dose-dependent inhibition of gland genesis though not as severe as either DES or tamoxifen. Coumestrol was about 103 more potent than equol as an estrogen and behaved much like DES with respect to its effects on uterine weight, glands, and ER levels. At the doses used in this study, equol failed to demonstrate either estrogenic or antiestrogenic activity.
新生小鼠接受香豆雌酚（一种植物雌激素）治疗后，阴道角化时间延长，并发生其他变化。
DOI：
10.1080/15287398509530635
发表时间：
1985
期刊：
Journal of toxicology and environmental health
影响因子：
--
作者：
Burroughs,CD;Bern,HA;Stokstad,EL
通讯作者：
Stokstad,EL