Pathogenesis of natural scrapie in sheep.
Pathogenesis of natural scrapie in sheep.
复制标题
绵羊天然瘙痒症的发病机制。
DOI：
--
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
M. Smits
中科院分区：
文献类型：
--
作者：
L. Keulen;B. Schreuder;M. Vromans;J. Langeveld;M. Smits
虽然羊瘙痒病作为绵羊和山羊的一种自然疾病已经被认识了很长时间，但其在自然宿主中的发病机制仍然不清楚。为了研究自然痒病的发病机制，我们使用免疫组化监测沉积的PrP（Sc）在各种组织中，收集在自然感染羊瘙痒症与PrP（VRQ）/PrP（VRQ）基因型，故意培育其自然痒病的短潜伏期。PrP（Sc）存在于所有动物的淋巴组织中，从5月龄开始。在这个年龄段，PrP（Sc）仅在十二指肠和回肠水平的肠神经系统（ENS）的神经组织中检测到。10月龄时，PrP（Sc）不仅见于ENS，还见于肠系膜颅侧/腹腔神经节、迷走神经背侧运动核和胸段T8-T10的中间外侧柱。PrP（Sc）在17月龄时首次在孤束核和结节神经节中检测到，在21月龄时在三叉神经节和几个脊神经节中检测到。由于羊瘙痒病剂主要由PrP（Sc）组成，如果不是完全由PrP（Sc）组成的话，这些结果表明ENS充当羊瘙痒病剂进入神经组织的门户，随后通过自主神经系统的交感神经和副交感神经传出纤维分别向脊髓和延髓进行向心和逆行扩散。PrP（Sc）在感觉神经节中的积累发生在CNS感染后，因此可能是由于羊瘙痒病病原体从CNS通过传入神经纤维的离心和顺行传播。
Although scrapie has been known for a long time as a natural disease of sheep and goats, the pathogenesis in its natural host still remains unclear. To study the pathogenesis of natural scrapie, we used immunohistochemistry to monitor the deposition of PrP(Sc) in various tissues, collected during a natural scrapie infection from sheep with the PrP(VRQ)/PrP(VRQ) genotype which were purposely bred for their short incubation period for natural scrapie. PrP(Sc) was present in the lymphoid tissues of all animals from the age of 5 months onwards. At this age, PrP(Sc) was detected in the neural tissues only in the enteric nervous system (ENS) at the level of the duodenum and ileum. At the age of 10 months, PrP(Sc) was not only found in the ENS but also in the ganglion mesentericum cranialis/coeliacum, the dorsal motor nucleus of the vagus, and the intermediolateral column of the thoracic segments T8-T10. PrP(Sc) was detected for the first time in the nucleus tractus solitarius and ganglion nodosus at 17 months of age and in the ganglion trigeminale and several spinal ganglia at 21 months of age. Since the scrapie agent consists largely, if not entirely of PrP(Sc), these results indicate that the ENS acts as a portal of entry to the neural tissues for the scrapie agent followed by centripetal and retrograde spread through sympathetic and parasympathetic efferent fibers of the autonomic nervous system to the spinal cord and medulla oblongata respectively. PrP(Sc) accumulation in sensory ganglia occurs after infection of the CNS and is therefore probably due to centrifugal and anterograde spread of the scrapie agent from the CNS through afferent nerve fibers.
DOI：
10.1152/ajpregu.1994.267.4.r974
发表时间：
1994-10-01
影响因子：
2.8
作者：
YATES, BJ;GRELOT, L;MILLER, AD
通讯作者：
MILLER, AD