The timely expression of myelin basic protein gene in cultured rat brain oligodendrocytes is independent of continuous neuronal influences
The timely expression of myelin basic protein gene in cultured rat brain oligodendrocytes is independent of continuous neuronal influences
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培养的大鼠脑少突胶质细胞中髓磷脂碱性蛋白基因的及时表达不依赖于持续的神经元影响
DOI：
10.1523/jneurosci.05-11-02955.1985
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发表时间：
1985
影响因子：
3.1
通讯作者：
R. Lazzarini
中科院分区：
文献类型：
--
作者：
N. Zeller;T. Behar;M. Dubois‐Dalcq;R. Lazzarini
采用免疫荧光和原位杂交技术研究了髓鞘碱性蛋白（MBP）基因在培养的大鼠少突胶质细胞中的发育表达。在只含有胶质细胞的新生大鼠脑培养物中，出生后5至6天，大量mbp特异性mRNA（通过射线自显像中的颗粒计数检测）突然在未成熟的少突胶质细胞中积累。MBP多在出生后6 ~ 8天出现；因此，一周后，出现了早期少突胶质细胞标记物半乳糖脑苷（GC）。MBP mRNA和MBP阳性细胞的百分比在出生后约15 d达到峰值，此后逐渐下降。在这些培养的少突胶质细胞中，MBP出现的时间似乎是在其发育的早期阶段确定的，并且独立于连续的神经元影响。培养的少突胶质细胞中MBP的出现时间与完整动物中MBP的出现时间有显著的对应关系。因此，从产前5天、新生儿和产后2天的动物制备的原代培养物都在大约相同的发育阶段表达MBP，即分别在培养14、8和6天后。此外，从脊髓中获得的培养少突胶质细胞比从中脑或半球获得的少突胶质细胞更早表达MBP，就像在完整的动物中一样。因此，在体外和体内，MBP基因的发育表达发生在相似的时间框架内。在少突胶质细胞富集的培养中，60%至80%的细胞表达MBP，与cDNA克隆的原位杂交显示，在细胞体中，有时在分化的少突胶质细胞过程中，存在MBP特异性mRNA。（摘要删节250字）
The developmental expression of the myelin basic protein (MBP) gene was studied in rat cultured oligodendrocytes using immunofluorescence and in situ hybridization. In newborn rat brain cultures, which contain only glial cells, large amounts of MBP-specific mRNA (as assayed by grain counts in autoradiograms) abruptly accumulated within immature oligodendrocytes 5 to 6 days postnatal. MBP always emerged 6 to 8 days after birth; thus, a week after, galactocerebroside (GC), an early oligodendrocyte marker, had appeared. The percentage of MBP mRNA and MBP-positive cells peaked at about 15 days postnatal and decreased thereafter. The time of emergence of MBP in these cultured oligodendrocytes appears to be determined at a very early stage in their development and independent of continuous neuronal influences. There is a striking correspondence between the times of appearance of MBP in cultured oligodendrocytes and those in the intact animal. Thus, primary cultures made from 5-day prenatal, newborn, and 2-day postnatal animals all express MBP at about the same developmental stage, namely, after 14, 8, and 6 days in culture, respectively. Furthermore, cultured oligodendrocytes obtained from the spinal cord express MBP before those obtained from midbrain or hemispheres, as they would in the intact animal. Thus, the developmental expression of the MBP gene occurs in a similar time frame in vitro and in vivo. In oligodendrocyte-enriched cultures, where 60% to 80% of the cells express MBP, in situ hybridization with the cDNA clone revealed MBP-specific mRNA in the cell body and sometimes in the processes of the differentiated oligodendrocytes.(ABSTRACT TRUNCATED AT 250 WORDS)
DOI：
10.1016/0165-3806(84)90056-7
发表时间：
1984-01-01
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
ECCLESTON, PA;SILBERBERG, DH
通讯作者：
SILBERBERG, DH
使用髓磷脂碱性蛋白 cDNA 克隆表征小鼠髓磷脂碱性蛋白信使 RNA。
DOI：
10.1073/pnas.81.1.18
发表时间：
1984
影响因子：
11.1
作者：
Zeller,NK;Hunkeler,MJ;Campagnoni,AT;Sprague,J;Lazzarini,RA
通讯作者：
Lazzarini,RA
豚鼠髓磷脂碱性蛋白肽 43-88 的表位：用单克隆抗体定位。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fritz,RB;Chou,CH
通讯作者：
Chou,CH