Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of Yersinia pestis.
Human Archaeological Dentin as Source of Polar and Less Polar Metabolites for Untargeted Metabolomic Research: The Case of Yersinia pestis.
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DOI：
10.3390/metabo13050588
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发表时间：
2023-04-24
期刊：
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
--
作者：
代谢组学的方法，如在活的个人的临床应用，已显示出潜在的用途，用于解决有关过去的问题时，适用于考古材料。在这里，我们研究的第一次的潜力，这种Omic的方法适用于从考古人类牙本质中提取的代谢物。牙本质从微量采样的受害者和非受害者的牙齿的牙髓鼠疫耶尔森氏菌（鼠疫）从第6世纪剑桥郡网站被用来评估潜在的使用这种独特的材料，通过液相色谱联用高分辨率质谱（LC-HRMS）的非靶向代谢组学研究疾病状态。结果表明，可能的内源性和外源性来源的小分子被保存在考古牙本质中的极性和极性较低/非极性代谢物的范围内;然而，在分析的小样本中，非靶向代谢物组学特征显示健康和感染个体之间没有明显的区别（n = 20）。本研究讨论了牙本质作为代谢组学测定小分子来源的潜力，并强调：（1）需要进行后续研究以优化采样方案，（2）需要更大样本量的研究，（3）需要更多数据库来放大考古科学中使用Omic技术可获得的积极结果。
Metabolomic approaches, such as in clinical applications of living individuals, have shown potential use for solving questions regarding the past when applied to archaeological material. Here, we study for the first time the potential of this Omic approach as applied to metabolites extracted from archaeological human dentin. Dentin obtained from micro sampling the dental pulp of teeth of victims and non-victims of Yersinia pestis (plague) from a 6th century Cambridgeshire site are used to evaluate the potential use of such unique material for untargeted metabolomic studies on disease state through liquid chromatography hyphenated to high-resolution mass spectrometry (LC-HRMS). Results show that small molecules of both likely endogenous and exogenous sources are preserved for a range of polar and less polar/apolar metabolites in archaeological dentin; however, untargeted metabolomic profiles show no clear differentiation between healthy and infected individuals in the small sample analysed (n = 20). This study discusses the potential of dentin as a source of small molecules for metabolomic assays and highlights: (1) the need for follow up research to optimise sampling protocols, (2) the requirements of studies with larger sample numbers and (3) the necessity of more databases to amplify the positive results achievable with this Omic technique in the archaeological sciences.
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DOI：
10.1073/pnas.1820447116
发表时间：
2019-06-18
影响因子：
11.1
作者：
Keller, Marcel;Spyrou, Maria A.;Krause, Johannes
通讯作者：
Krause, Johannes
影响因子：
4.1
作者：
Aderemi AV;Ayeleso AO;Oyedapo OO;Mukwevho E
通讯作者：
Mukwevho E
影响因子：
7.7
作者：
Demarchi, Beatrice;Hall, Shaun;Collins, Matthew J.
通讯作者：
Collins, Matthew J.
影响因子：
1.3
作者：
EVERSHED, RP
通讯作者：
EVERSHED, RP
影响因子：
0.6
作者：
Manifold, B. M.
通讯作者：
Manifold, B. M.