Painting the chromosomes of Brachypodium: current status and future prospects.

Painting the chromosomes of Brachypodium: current status and future prospects.
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DOI:
10.1007/s00412-011-0326-9
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发表时间:
2011-10
期刊:
影响因子:
1.6
通讯作者:
Hasterok R
Hasterok R
中科院分区:
生物学3区
文献类型:
--
作者:
Idziak D;Betekhtin A;Wolny E;Lesniewska K;Wright J;Febrer M;Bevan MW;Jenkins G;Hasterok R

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染色体彩绘是现代分子细胞遗传学中最强大和最壮观的工具之一,可以对核基因组结构和进化进行复杂的分析。多年来,这项技术仅限于哺乳动物染色体的研究,因为它未能在植物基因组中工作,主要是由于存在大量的重复DNA,这些DNA是所有互补染色体所共有的。有序的,染色体特异性BAC克隆的拟南芥含有相对较少的重复基因组DNA的可用性,使第一个双子叶植物染色体彩绘。在这里,我们第一次展示了单子叶植物的三种不同细胞型的染色体绘画-模式草,二穗短柄草。提出了进一步深入研究的可能方向,如禾本科植物核型的演化、减数分裂染色体的行为和间期染色体分布的分析。
Chromosome painting is one of the most powerful and spectacular tools of modern molecular cytogenetics, enabling complex analyses of nuclear genome structure and evolution. For many years, this technique was restricted to the study of mammalian chromosomes, as it failed to work in plant genomes due mainly to the presence of large amounts of repetitive DNA common to all the chromosomes of the complement. The availability of ordered, chromosome-specific BAC clones of Arabidopsis thaliana containing relatively little repetitive genomic DNA enabled the first chromosome painting in dicotyledonous plants. Here, we show for the first time chromosome painting in three different cytotypes of a monocotyledonous plant—the model grass, Brachypodium distachyon. Possible directions of further detailed studies are proposed, such as the evolution of grass karyotypes, the behaviour of meiotic chromosomes, and the analysis of chromosome distribution at interphase.
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发表时间: 2010-10-18
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