An integrated physical, genetic and cytogenetic map of Brachypodium distachyon, a model system for grass research.
An integrated physical, genetic and cytogenetic map of Brachypodium distachyon, a model system for grass research.
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DOI：
10.1371/journal.pone.0013461
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发表时间：
2010-10-18
期刊：
影响因子：
3.7
通讯作者：
Bevan MW
中科院分区：
文献类型：
--
作者：
Febrer M;Goicoechea JL;Wright J;McKenzie N;Song X;Lin J;Collura K;Wissotski M;Yu Y;Ammiraju JS;Wolny E;Idziak D;Betekhtin A;Kudrna D;Hasterok R;Wing RA;Bevan MW
禾本科植物亚科包括一些最重要的农作物、饲料和草坪物种，例如小麦、大麦和黑麦草。开发基因组资源，例如全基因组物理图谱，用于分析这些作物的大型复杂基因组并促进草类生物学研究，是植物生物学的一个重要目标。我们描述了一种基于细菌人工染色体 (BAC) 的野生普类草 Brachypodium distachyon 的物理图谱，并使用 BAC 末端序列 (BES) 将其与全基因组鸟枪序列 (WGS) 组装进行整合。生成的物理图谱包含跨越 272 Mb 基因组的 26 个重叠群。物理图谱中的 BES 也被用来整合遗传图谱。这为已发布的 WGS 组装提供了独立的验证和确认。映射的 BAC 用于荧光原位杂交 (FISH) 实验，以高分辨率将集成的物理图谱和序列组件与染色体对齐。物理、遗传和细胞遗传学图谱与全基因组鸟枪序列组装相结合，提高了这一重要基因组序列的准确性和耐久性，并将直接促进基因分离。
The pooid subfamily of grasses includes some of the most important crop, forage and turf species, such as wheat, barley and Lolium. Developing genomic resources, such as whole-genome physical maps, for analysing the large and complex genomes of these crops and for facilitating biological research in grasses is an important goal in plant biology. We describe a bacterial artificial chromosome (BAC)-based physical map of the wild pooid grass Brachypodium distachyon and integrate this with whole genome shotgun sequence (WGS) assemblies using BAC end sequences (BES). The resulting physical map contains 26 contigs spanning the 272 Mb genome. BES from the physical map were also used to integrate a genetic map. This provides an independent vaildation and confirmation of the published WGS assembly. Mapped BACs were used in Fluorescence In Situ Hybridisation (FISH) experiments to align the integrated physical map and sequence assemblies to chromosomes with high resolution. The physical, genetic and cytogenetic maps, integrated with whole genome shotgun sequence assemblies, enhance the accuracy and durability of this important genome sequence and will directly facilitate gene isolation.
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