Kre28-Spc105 interaction is essential for Spc105 loading at the kinetochore.
Kre28-Spc105 interaction is essential for Spc105 loading at the kinetochore.
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DOI：
10.1098/rsob.210274
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发表时间：
2022-01
期刊：
影响因子：
5.8
通讯作者：
Joglekar AP
中科院分区：
文献类型：
--
作者：
Roy B;Sim J;Han SJY;Joglekar AP
动粒（KT）是将姐妹染色单体连接到纺锤体微管（MT）上的大分子蛋白质组装体，并在有丝分裂过程中介导染色体的精确分离。外部KT由KMN网络组成，KMN网络是一种蛋白质超复合物，包含Knl 1（酵母Spc 105）、Mis 12（酵母Mtw 1）和Ndc 80（酵母Ndc 80），其含有MT结合位点。在KMN网络内，Spc 105充当参与纺锤体组装检查点（SAC）信号传导的组件的交互枢纽。已知Spc 105与KT组分Kre 28形成复合物。然而，Kre 28在出芽酵母KT中的物理定位尚不清楚。Kre 28在KT的确切功能也是未知的。在这里，我们调查Spc 105和Kre 28如何相互作用，以及它们是如何组织在双向酵母KT使用遗传学和细胞生物学实验。我们的显微镜数据显示，Spc 105和Kre 28定位在KT与1：1的化学计量。我们还表明，Kre 28-Spc 105的相互作用是重要的Spc 105蛋白质的周转和必不可少的相互招聘在KT。我们创建了几个截断突变体的kre 28影响Spc 105加载在KT。当过表达时，这些突变体维持细胞活力，但SAC信号传导和KT双向性受损。因此，我们得出结论，Kre 28有助于染色体双向和高保真分离至少间接调节Spc 105定位在KT。
Kinetochore (KTs) are macromolecular protein assemblies that attach sister chromatids to spindle microtubules (MTs) and mediate accurate chromosome segregation during mitosis. The outer KT consists of the KMN network, a protein super-complex comprising Knl1 (yeast Spc105), Mis12 (yeast Mtw1), and Ndc80 (yeast Ndc80), which harbours sites for MT binding. Within the KMN network, Spc105 acts as an interaction hub of components involved in spindle assembly checkpoint (SAC) signalling. It is known that Spc105 forms a complex with KT component Kre28. However, where Kre28 physically localizes in the budding yeast KT is not clear. The exact function of Kre28 at the KT is also unknown. Here, we investigate how Spc105 and Kre28 interact and how they are organized within bioriented yeast KTs using genetics and cell biological experiments. Our microscopy data show that Spc105 and Kre28 localize at the KT with a 1 : 1 stoichiometry. We also show that the Kre28–Spc105 interaction is important for Spc105 protein turn-over and essential for their mutual recruitment at the KTs. We created several truncation mutants of kre28 that affect Spc105 loading at the KTs. When over-expressed, these mutants sustain the cell viability, but SAC signalling and KT biorientation are impaired. Therefore, we conclude that Kre28 contributes to chromosome biorientation and high-fidelity segregation at least indirectly by regulating Spc105 localization at the KTs.
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影响因子：
21.3
作者：
Aravamudhan P;Goldfarb AA;Joglekar AP
通讯作者：
Joglekar AP
影响因子：
4.2
作者：
Musacchio A;Desai A
通讯作者：
Desai A
双重机制调节纺锤体组装检查点蛋白募集到萌芽的酵母菌。
影响因子：
3.3
作者：
Aravamudhan P;Chen R;Roy B;Sim J;Joglekar AP
通讯作者：
Joglekar AP
DOI：
10.1016/j.cub.2009.02.056
发表时间：
2009-04-28
期刊：
Current biology : CB
影响因子：
--
作者：
Joglekar AP;Bloom K;Salmon ED
通讯作者：
Salmon ED
影响因子：
4.5
作者：
Akiyoshi B;Nelson CR;Duggan N;Ceto S;Ranish JA;Biggins S
通讯作者：
Biggins S