Identification and characterization of the peroxin 1 gene MoPEX1 required for infection-related morphogenesis and pathogenicity in Magnaporthe oryzae.
Identification and characterization of the peroxin 1 gene MoPEX1 required for infection-related morphogenesis and pathogenicity in Magnaporthe oryzae.
复制标题
稻瘟病菌感染相关形态发生和致病性所需的过氧化物酶 1 基因 MoPEX1 的鉴定和表征
DOI：
10.1038/srep36292
复制
发表时间：
2016-11-08
影响因子：
4.6
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Deng S;Gu Z;Yang N;Li L;Yue X;Que Y;Sun G;Wang Z;Wang J
过氧化物酶体是许多植物病原真菌致病所必需的，但真菌致病性与过氧化物酶体功能之间的关系尚不完全清楚。本文报道了稻瘟病菌T-DNA插入突变体C445的鉴定，该突变体具有致病性缺陷。对突变的分析证实了MoPEX 1基因的插入，MoPEX 1基因编码一种推定的过氧化物酶1同源物。MoPEX 1的靶基因缺失突变体是非致病性的，并且在营养生长、分生孢子和附着胞形成中受损。Δ Mopex 1突变体形成的附着胞异常、着色少、无功能，但不能穿透植物角质层。Δ Mopex 1突变体在脂肪酸利用方面存在缺陷（例如，橄榄油和吐温-20）。此外，MoPEX 1的缺失显着削弱了附着胞发育过程中脂滴的动员和降解。有趣的是，MoPEX 1的缺失阻断了过氧化物酶体基质蛋白的输入。分析了M。表达GFP-MoPEX 1和RFP-PTS 1融合蛋白的大肠杆菌菌株显示MoPex 1定位于过氧化物酶体。酵母双杂交实验表明，MoPex 1物理相互作用与MoPex 6，过氧化物酶体基质蛋白的真菌形态发生和致病性的重要。综上所述，我们得出结论，MoPEX 1在过氧化物酶体功能中起重要作用，并且是M.米。
Peroxisomes are required for pathogenicity in many phytopathogenic fungi, but the relationships between fungal pathogenicity and peroxisomal function are not fully understood. Here, we report the identification of a T-DNA insertional mutant C445 of Magnaporthe oryzae, which is defective in pathogenicity. Analysis of the mutation confirmed an insertion into the gene MoPEX1, which encodes a putative homologue to peroxin 1. Targeted gene deletion mutants of MoPEX1 were nonpathogenic and were impaired in vegetative growth, conidiation, and appressorium formation. ΔMopex1 mutants formed abnormal, less pigmented, and nonfunctional appressoria, but they were unable to penetrate plant cuticle. The ΔMopex1 mutants were defective in the utilization of fatty acids (e.g., olive oil and Tween-20). Moreover, deletion of MoPEX1 significantly impaired the mobilization and degradation of lipid droplets during appressorium development. Interestingly, deletion of MoPEX1 blocked the import of peroxisomal matrix proteins. Analysis of an M. oryzae strain expressing GFP-MoPEX1 and RFP-PTS1 fusions revealed that MoPex1 localizes to peroxisomes. Yeast two hybrid experiments showed that MoPex1 physically interacts with MoPex6, a peroxisomal matrix protein important for fungal morphogenesis and pathogenicity. Taken together, we conclude that MoPEX1 plays important roles in peroxisomal function and is required for infection-related morphogenesis and pathogenicity in M. oryzae.
登录
查看更多内容
DOI：
10.1083/jcb.135.1.1
发表时间：
1996-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Distel B;Erdmann R;Gould SJ;Blobel G;Crane DI;Cregg JM;Dodt G;Fujiki Y;Goodman JM;Just WW;Kiel JA;Kunau WH;Lazarow PB;Mannaerts GP;Moser HW;Osumi T;Rachubinski RA;Roscher A;Subramani S;Tabak HF;Tsukamoto T;Valle D;van der Klei I;van Veldhoven PP;Veenhuis M
通讯作者：
Veenhuis M
DOI：
10.1111/j.1749-6632.1982.tb21415.x
发表时间：
1982-01-01
影响因子：
5.2
作者：
HAJRA, AK;BISHOP, JE
通讯作者：
BISHOP, JE
影响因子：
2.6
作者：
GOULD, SJ;MCCOLLUM, D;SUBRAMANI, S
通讯作者：
SUBRAMANI, S
影响因子：
2.6
作者：
KRAUSE, T;KUNAU, WH;ERDMANN, R
通讯作者：
ERDMANN, R
影响因子：
3
作者：
Money, NP;Howard, RJ
通讯作者：
Howard, RJ