Cancer-specific mRNAs in thyroid carcinomas: detection, use, and their implication in thyroid carcinogenesis.
Cancer-specific mRNAs in thyroid carcinomas: detection, use, and their implication in thyroid carcinogenesis.
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甲状腺癌中的癌症特异性 mRNA：检测、使用及其在甲状腺癌发生中的意义。
DOI：
10.1507/endocrj.49.97
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发表时间：
2002
影响因子：
2
通讯作者：
N. Amino
中科院分区：
文献类型：
--
作者：
T. Takano;N. Amino
通过利用癌组织中以限制性方式表达的特异性mRNA，可以更容易地建立基于分子的甲状腺癌诊断。因此，利用序列特异性差异显示（SS-DD）、基因表达系列分析（SAGE）等新技术，在甲状腺癌中鉴定了几种癌特异性mRNA。利用这些肿瘤特异性mRNA，已经发展了一些甲状腺癌术前诊断的新方法。在一种这样的方法中，抽吸活检-逆转录-聚合酶链反应（ABRP），从用于细针抽吸活检（FNAB）的针内的剩余细胞中提取RNA，从而允许同时进行基于细胞学和分子的诊断。ABRP提供了RNA信息和细胞学诊断，而不会进一步侵入患者。ABRP检测肿瘤特异性mRNA，可对乳头状癌、间变性癌、髓样癌及部分恶性淋巴瘤作出术前准确诊断。为什么癌症特异性mRNA，特别是那些在胎儿组织中过表达的mRNA，可以清楚地区分良性组织和癌症，而基因组改变，如RAS或P53基因的突变则不能，这一点还有待澄清。此外，广泛接受的多步骤致癌假说不能解释甲状腺癌的一些临床和实验结果。考虑到这些事实，我们提出了一种新的甲状腺癌发生假说，即“生殖细胞致癌”假说，即癌细胞来源于胎儿甲状腺生殖细胞（成甲状腺细胞）的残余物，而不是正常的甲状腺滤泡细胞。
Molecular-based diagnosis ofthyroid carcinomas can be more easily establishedby utilizing specific mRNAs that are expressed in a restricted manner in cancer tissues. Accordingly, several cancer-specific mRNAs in thyroid carcinomas have been identified by means of sequence specific-differential display (SS-DD), serial analysis of gene expression (SAGE) and other new techniques. By using these cancer-specific mRNAs, some new methods of preoperative diagnosis of thyroid carcinomas have been developed. In one such method, Aspiration Biopsy-Reverse Transcription-Polymerase Chain Reaction (ABRP), RNA is extracted from leftover cells within the needle used for fine needle aspiration biopsies (FNABs), thereby allowing cytological and molecular-based diagnoses to be performed simultaneously. ABRP provides both RNA information and a cytological diagnosis without further invasion to the patient. By ABRP detection of cancer-specific mRNAs, papillary, anaplastic and medullary carcinomas and a part of malignant lymphomas can be accurately diagnosed preoperatively. It remains to be clarified why cancer-specific mRNAs, especially those that are overexpressed in fetal tissues, can clearly distinguish benign tissues from carcinomas, while genomic alternations, such as mutations in the RAS or P53 gene cannot. Further, the widely accepted hypothesis of multi-step carcinogenesis cannot explain some of the clinical and experimental findings of thyroid carcinomas. Considering these facts, we propose a novel hypothesis of thyroid carcinogenesis, the "germ-cell carcinogenesis" hypothesis, in which cancer cells derive from the remnants of fetal thyroid germ cells (thyroblasts) instead of normal thyroid follicular cells.
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通过外周血中甲状腺球蛋白信使核糖核酸的扩增对残留和复发性甲状腺癌进行分子诊断。
DOI：
10.1210/jcem.83.12.5346
发表时间：
1998
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
--
作者：
Ringel,MD;Ladenson,PW;Levine,MA
通讯作者：
Levine,MA
影响因子：
4.8
作者：
S. Jhiang;J. Sagartz;Q. Tong;J. Parker-Thornburg;C. Capen;Je-Yoel Cho;Shunhua Xing;C. Ledent
通讯作者：
S. Jhiang;J. Sagartz;Q. Tong;J. Parker-Thornburg;C. Capen;Je-Yoel Cho;Shunhua Xing;C. Ledent
影响因子：
158.5
作者：
VOGELSTEIN, B;FEARON, ER;BOS, JL
通讯作者：
BOS, JL
影响因子：
15.9
作者：
FAGIN, JA;MATSUO, K;KOEFFLER, HP
通讯作者：
KOEFFLER, HP