Therapeutic Antitumor Efficacy of Cancer Stem Cell-Derived DRibble Vaccine on Colorectal Carcinoma.
Therapeutic Antitumor Efficacy of Cancer Stem Cell-Derived DRibble Vaccine on Colorectal Carcinoma.
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癌症干细胞衍生的 Dribble 疫苗对结直肠癌的抗肿瘤疗效
DOI：
10.7150/ijms.61510
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发表时间：
2021
影响因子：
3.6
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Fu C;Tian G;Duan J;Liu K;Zhang C;Yan W;Wang Y
Dendritic cell (DC)-based immunotherapy has been a promising strategy for colon cancer therapy, but the efficacy of dendritic cell vaccines is in part limited by immunogenicity of loaded antigens. In this study, we aimed to identify a putative tumor antigen that can generate or enhance anti-tumor immune responses against colon cancer. CD44+ colon cancer stem cells (CCSCs) were isolated from mouse colorectal carcinoma CT-26 cell cultures and induced to form defective ribosomal products-containing autophagosome-rich blebs (DRibbles) by treatment with rapamycin, bortezomib, and ammonium chloride. DRibbles were characterized by western blot and transmission electron microscopy. DCs generated from the mice bone marrow monocytes were cocultured with DRibbles, then surface markers of DCs were analyzed by flow cytometry. Meanwhile, the efficacy of DRibble-DCs was examined in vivo. Our results showed that CCSC-derived DRibbles upregulated CD80, CD86, major histocompatibility complex (MHC)-I, and MHC-II on DCs and induced proliferation of mouse splenic lymphocytes and CD8+ T cells. In a model of colorectal carcinoma using BALB/c mice with robust tumor growth and mortality, DC vaccine pulsed with CCSC-derived DRibbles suppressed tumor growth and extended survival. A lactate dehydrogenase test indicated a strong cytolytic activity of cytotoxic T-cells derived from mice vaccinated with CCSC-derived DRibbles against CT-26 cells. Furthermore, flow cytometry analyses showed that the percentages of IFN-γ-producing CD8+ T-cells were increased in SD-DC group compare with the other groups. These findings provide a rationale for novel immunotherapeutic anti-tumor approaches based on DRibbles derived from colon cancer stem cells.
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影响因子：
7.5
作者：
Salem, Mohamed L.;Nassef, Mohamed;Essa, Ibrahim
通讯作者：
Essa, Ibrahim
CD28在T细胞稳态中共同刺激：最近的观点。
影响因子：
7.2
作者：
Beyersdorf N;Kerkau T;Hünig T
通讯作者：
Hünig T
自噬体生物发生分解为不同的成核和膨胀步骤。
DOI：
10.1083/jcb.151.5.1025
发表时间：
2000-11-27
期刊：
The Journal of cell biology
影响因子：
--
作者：
Abeliovich H;Dunn WA Jr;Kim J;Klionsky DJ
通讯作者：
Klionsky DJ
DOI：
10.1073/pnas.95.16.9482
发表时间：
1998-08-04
影响因子：
11.1
作者：
Fields, RC;Shimizu, K;Mulé, JJ
通讯作者：
Mulé, JJ
影响因子：
32.4
作者：
Jeannin, P;Magistrelli, G;Delneste, Y
通讯作者：
Delneste, Y