Coherency image analysis to quantify collagen architecture: implications in scar assessment.
Coherency image analysis to quantify collagen architecture: implications in scar assessment.
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DOI：
10.1039/c7ra12693j
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发表时间：
2018-03-05
期刊：
影响因子：
3.9
通讯作者：
Iyer, K. Swaminathan
中科院分区：
文献类型：
--
作者：
Clemons, T. D.;Bradshaw, M.;Toshniwal, P.;Chaudhari, N.;Stevenson, A. W.;Lynch, J.;Fear, M. W.;Wood, F. M.;Iyer, K. Swaminathan
正常、未损伤的真皮和瘢痕组织（例如在瘢痕疙瘩中发现的瘢痕组织）之间的重要组织学差异是胶原沉积和排列的模式（形态结构）。在未受伤的真皮中，胶原束结构似乎是随机组织的（或呈篮状编织形成），而在病理条件下，如瘢痕疙瘩瘢痕组织中，胶原束通常呈螺旋状或在营养不良的瘢痕中，胶原以平行构型更密集地堆积。就皮肤而言，疤痕会使真皮功能丧失，使其变得更弱、僵硬并失去最佳功能。胶原蛋白取向的客观和可量化评估的缺乏是监测瘢痕治疗进展的主要瓶颈。在这篇文章中，一个新的定量分析胶原蛋白的取向的方法。该方法使用由模型瘢痕环境中的细胞产生的胶原来证明，并在体外检查TGF β刺激后的胶原重塑。该方法被证明是可靠和有效的，在识别显着的一致性差异，胶原蛋白沉积的人瘢痕疙瘩疤痕细胞。该技术还比较分析胶原蛋白结构在大鼠切片的正常，瘢痕皮肤和肌腱组织。结果表明，所提出的计算方法提供了一种快速和强大的方式分析胶原蛋白的取向超过现有的方法。本研究将该方法确立为体外和组织治疗模式监测的初步手段，这些治疗模式预计会改变胶原形态。一种用于瘢痕形成后胶原蛋白结构的快速和稳健定量的新技术。
An important histological difference between normal, uninjured dermis and scar tissue such as that found in keloid scars is the pattern (morphological architecture) in which the collagen is deposited and arranged. In the uninjured dermis, collagen bundle architecture appears randomly organized (or in a basket weave formation), whereas in pathological conditions such as keloid scar tissue, collagen bundles are often found in whorls or in a hypotrophic scar collagen is more densely packed in a parallel configuration. In the case of skin, a scar disables the dermis, leaving it weaker, stiff and with a loss of optimal functionality. The absence of objective and quantifiable assessments of collagen orientation is a major bottleneck in monitoring progression of scar therapeutics. In this article, a novel quantitative approach for analyzing collagen orientation is reported. The methodology is demonstrated using collagen produced by cells in a model scar environment and examines collagen remodeling post-TGFβ stimulation in vitro. The method is shown to be reliable and effective in identifying significant coherency differences in the collagen deposited by human keloid scar cells. The technique is also compared for analysing collagen architecture in rat sections of normal, scarred skin and tendon tissue. Results demonstrate that the proposed computational method provides a fast and robust way of analyzing collagen orientation in a manner surpassing existing methods. This study establishes this methodology as a preliminary means of monitoring in vitro and in tissue treatment modalities which are expected to alter collagen morphology. A novel technique for the fast and robust quantification of collagen architecture following scarring.
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影响因子：
14
作者：
Kador, Karl E.;Montero, Ramon B.;Venugopalan, Praseeda;Hertz, Jonathan;Zindell, Allison N.;Valenzuela, Daniel A.;Uddin, Mohammed S.;Lavik, Erin B.;Muller, Kenneth J.;Andreopoulos, Fotios M.;Goldberg, Jeffrey L.
通讯作者：
Goldberg, Jeffrey L.
影响因子：
1.4
作者：
Forbes-Duchart, Lisa;Marshall, Sheryle;Cooper, Juliette E.
通讯作者：
Cooper, Juliette E.
影响因子：
7.7
作者：
EblenZajjur, A;Salas, R;Vanegas, H
通讯作者：
Vanegas, H
影响因子：
3.5
作者：
Rezakhaniha, R.;Agianniotis, A.;Stergiopulos, N.
通讯作者：
Stergiopulos, N.
影响因子：
5
作者：
de Vries, HJC;Enomoto, DNH;Bos, JD
通讯作者：
Bos, JD