Mangrove trees affect the community structure and distribution of anammox bacteria at an anthropogenic-polluted mangrove in the Pearl River Delta reflected by 16S rRNA and hydrazine oxidoreductase (HZO) encoding gene analyses.
Mangrove trees affect the community structure and distribution of anammox bacteria at an anthropogenic-polluted mangrove in the Pearl River Delta reflected by 16S rRNA and hydrazine oxidoreductase (HZO) encoding gene analyses.
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DOI：
10.1007/s10646-011-0711-4
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发表时间：
2011-11
期刊：
影响因子：
2.7
通讯作者：
Gu, Ji-Dong
中科院分区：
文献类型：
--
作者：
Li, Meng;Hong, Yi-Guo;Cao, Hui-Luo;Gu, Ji-Dong
对珠江三角洲某污染地区红树林沉积物表层（1 ~ 2 cm）和下层（20 ~ 21 cm）的厌氧氨氧化（anammox）细菌群落结构进行了研究，研究地点分别为离红树林近（S1）、10 m和1000 m （S2）的红树林沉积物。在50℃时，厌氧氨氧化菌的16S rRNA和肼氧化还原酶（hydrazine oxidoreductase， HZO）编码基因在下层沉积物中均表现出较高的多样性，但在红树林下层沉积物中未检测到。S1和S2在表层和下层厌氧氨氧化菌群相似，与S0有较大差异。3个地点的厌氧氨氧化菌丰富度表层均高于下层；16S rRNA基因显示厌氧氨氧化菌由隶属于Scalindua属的4个系统发育群组成，1个群与潜在的厌氧氨氧化菌有关；hzo基因显示，在红树林沉积物中除了发现与“Scalindua”相关的序列外，还发现了与“Kuenenia”、“Brocadia”和“Jettenia”相关的序列。hzo基因丰度在下层沉积物中从36.5 × 104拷贝/g干底泥下降到11.0 × 104拷贝/g干底泥，在下层沉积物中从低于检测限增加到31.5 × 104拷贝/g干底泥。结果表明，厌氧氨氧化菌群落可能受到红树林的强烈影响。此外，相关分析表明，沉积物中的氧化还原电位和铵与亚硝酸盐的摩尔比可能是影响红树林沉积物中厌氧氨氧化菌多样性和分布的重要因素。本文的在线版本（doi:10.1007/s10646-011-0711-4）包含补充材料，仅供授权用户使用。
Anaerobic ammonium oxidizing (anammox) bacterial community structures were investigated in surface (1–2 cm) and lower (20–21 cm) layers of mangrove sediments at sites located immediately to the mangrove trees (S0), 10 m (S1) and 1000 m (S2) away from mangrove trees in a polluted area of the Pearl River Delta. At S0, both 16S rRNA and hydrazine oxidoreductase (HZO) encoding genes of anammox bacteria showed high diversity in lower layer sediments, but they were not detectable in lower layer sediments in mangrove forest. S1 and S2 shared similar anammox bacteria communities in both surface and lower layers, which were quite different from that of S0. At all three locations, higher richness of anammox bacteria was detected in the surface layer than the lower layer; 16S rRNA genes revealed anammox bacteria were composed by four phylogenetic clusters affiliated with the “Scalindua” genus, and one group related to the potential anammox bacteria; while the hzo genes showed that in addition to sequences related to the “Scalindua”, sequences affiliated with genera of “Kuenenia”, “Brocadia”, and “Jettenia” were also detected in mangrove sediments. Furthermore, hzo gene abundances decreased from 36.5 × 104 to 11.0 × 104 copies/gram dry sediment in lower layer sediments while increased from below detection limit to 31.5 × 104 copies/gram dry sediment in lower layer sediments from S0 to S2. The results indicated that anammox bacteria communities might be strongly influenced by mangrove trees. In addition, the correlation analysis showed the redox potential and the molar ratio of ammonium to nitrite in sediments might be important factors affecting the diversity and distribution of anammox bacteria in mangrove sediments. The online version of this article (doi:10.1007/s10646-011-0711-4) contains supplementary material, which is available to authorized users.
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影响因子：
1.4
作者：
Risgaard-Petersen, N;Meyer, RL;Revsbech, NP
通讯作者：
Revsbech, NP
影响因子：
64.8
作者：
Kuypers, MMM;Sliekers, AO;Jetten, MSM
通讯作者：
Jetten, MSM
影响因子：
3.6
作者：
Rich, Jeremy J.;Dale, Olivia R.;Ward, Bess B.
通讯作者：
Ward, Bess B.
影响因子：
3.4
作者：
Kartal, Boran;Rattray, Jayne;Strous, Marc
通讯作者：
Strous, Marc
海洋沉积物中厌氧氨氧化菌16S rRNA和肼氧化还原酶基因检测引物组比较
影响因子：
5
作者：
Li, Meng;Hong, Yiguo;Gu, Ji-Dong
通讯作者：
Gu, Ji-Dong