Application of the Resazurin Cell Viability Assay to Monitor Escherichia coli and Salmonella Typhimurium Inactivation Mediated by Phages.
Application of the Resazurin Cell Viability Assay to Monitor Escherichia coli and Salmonella Typhimurium Inactivation Mediated by Phages.
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DOI：
10.3390/antibiotics10080974
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发表时间：
2021-08-12
期刊：
影响因子：
--
通讯作者：
Almeida A
中科院分区：
文献类型：
--
作者：
Costa P;Gomes ATPC;Braz M;Pereira C;Almeida A
使用噬菌体（或噬菌体）的细菌灭活已经成为细菌感染的有效解决方案，但是用于评估噬菌体对细菌的有效性的筛选方法不够快速、可靠或精确。通过计数菌落形成单位（CFU）（一种费力且耗时的方法）或通过监测光密度（OD）（一种不太敏感的方法）监测细菌浓度，评价了细菌灭活的效率。本研究采用刃天青细胞活力测定法对不同菌种在不同灭菌剂灭活过程中的存活率进行了监测，并与标准方法进行了比较。结果表明，刃天青比色法测定细胞活力的结果与标准的菌落计数和给予法相似，且比OD法灵敏度高。刃天青试验可用于同时使用两种不同的细菌菌株和几种不同的细菌快速获得细胞活力效应曲线的结果，这在筛选研究中是非常有价值的。此外，该方法被确立为一种有效的，准确的和快速的方法相比，广泛用于监测细菌灭活介导的细菌。
Bacterial inactivation using bacteriophages (or phages) has emerged as an effective solution for bacterial infections, but the screening methods used to evaluate the effectiveness of the phages to inactivate bacteria are not fast, reliable or precise enough. The efficiency of bacterial inactivation by phages has been evaluated by monitoring bacterial concentration either by counting colony-forming units (CFU), a laborious and time-consuming method, or by monitoring the optical density (OD), a less sensitive method. In this study, the resazurin cell viability assay was used to monitor the viability of bacteria from different genera during the inactivation by different phages, and the results were compared with the standard methods used to assess bacterial inactivation. The results showed that the resazurin colorimetric cell viability assay produces similar results to the standard method of colony-counting and giving, and also more sensitive results than the OD method. The resazurin assay can be used to quickly obtain the results of the cell viability effect profile using two different bacterial strains and several different phages at the same time, which is extremely valuable in screening studies. Moreover, this methodology is established as an effective, accurate and rapid method when compared to the ones widely used to monitor bacterial inactivation mediated by phages.
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影响因子：
4.5
作者：
Costa, Pedro;Pereira, Carla;Almeida, Adelaide
通讯作者：
Almeida, Adelaide
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Yano, T
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1.9
作者：
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Kuhl, S.
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作者：
Bicalho, R. C.;Santos, T. M. A.;Machado, V. S.
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Machado, V. S.