Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices.
Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices.
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生物分子基序的均匀对齐生物共轭，用于集成到微制造的微流体装置中。
DOI：
10.1016/j.ab.2012.02.020
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发表时间：
2012
影响因子：
2.9
通讯作者：
Braddick LM
中科院分区：
文献类型：
--
作者：
Braddick LM
完整的细节和一步一步的指导适合打印蛋白质对准微米尺寸的传感器和随后的集成和对准的微流体结构。使用一种新型的半自动微接触打印机实现了微米级生物分子图案与底层基底在预定位置的精确对准和接枝。通过在x、y和z方向上集成光学对准方法，实现了微米级印模的均匀接触。通过在接触期间对印模的精细控制来避免印模的特征压缩。这种印刷方法已经与稳健的、相容的生物缀合物化学结合开发，用于用NeutrAvidin-“着墨”印模图案化葡聚糖官能化的氧化硅基底，随后与生物素官能化的蛋白质孵育。生物缀合物化学使得获得蛋白质（没有变性）在印刷基序上的均匀覆盖，并且实现初始掩模形状和尺寸的再现。随后还描述了与微流体结构的集成，该微流体结构与基板上的印刷图案对准。
Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined locations is achieved using a novel semi-automated microcontact printer. Through integration of optical alignment methods in the x, y, and z directions, uniform contact of micron-sized stamps is achieved. Feature compression of the stamp is avoided by fine control of the stamp during contact. This printing method has been developed in combination with robust, compatible bioconjugate chemistry for patterning of a dextran-functionalized silicon oxide substrate with a NeutrAvidin-“inked” stamp and subsequent incubation with a biotin-functionalized protein. The bioconjugate chemistry is such that uniform coverage of the protein (without denaturation) over the printed motif is obtained and reproduction of the initial mask shape and dimensions is achieved. Later integration with a microfluidic structure aligned with the printed motif on the substrate is also described.
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