Liposome surface functionalization based on different anchoring lipids via Staudinger ligation.
Liposome surface functionalization based on different anchoring lipids via Staudinger ligation.
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DOI：
10.1039/c3ob41721b
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发表时间：
2014-02-28
影响因子：
3.2
通讯作者：
Sun XL
中科院分区：
文献类型：
--
作者：
Vabbilisetty P;Sun XL
Liposome surface functionalization facilitates enormous potential applications of liposomes, such as enhanced stability, bioactive liposome conjugates, and targeted drug, gene and image agent delivery. Anchoring lipids are needed for grafting ligands of interest and play important roles in ligands grafting density, liposome stability, and liposome chemical and physical characteristics as well. In this report, glyco-functionalized liposome systems based on two kinds of anchoring lipid, phosphatidylethonalamine (PE) and cholesterol (Chol) were prepared by post chemically selective functionalization via Staudinger ligation. The size and stability of the liposomes were confirmed by dynamic light scattering (DLS). Particularly, the impact of anchor lipids on the stability of glyco-functionalized liposomes was investigated by comparing two different anchor lipids, namely Chol-PEG2000-TP and DSPE-PEG2000-TP. In addition, the encapsulation and releasing capacity of the glycosylated liposome based on the two anchoring lipids were investigated by entrapping 5, 6-carboxyfluorescein (CF) dye and monitoring the fluorescence leakage, respectively. Furthermore, the density and accessibility of grafted carbohydrate residues on the liposome surface were evaluated for the two anchoring lipids-derived liposomes with lectin binding, respectively.
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DOI：
10.1016/0005-2736(91)90246-5
发表时间：
1991-07-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ALLEN, TM;HANSEN, C;YAUYOUNG, A
通讯作者：
YAUYOUNG, A
影响因子：
4.7
作者：
Nakano, Y;Mori, M;Uchida, T
通讯作者：
Uchida, T
影响因子：
4.7
作者：
Hassane, Fatouma Said;Frisch, Benoit;Schuber, Francis
通讯作者：
Schuber, Francis
影响因子：
4.7
作者：
Pan, Xiaogang;Wu, Gong;Lee, Robert J.
通讯作者：
Lee, Robert J.
影响因子：
3.7
作者：
Song S;Cheong LZ;Falkeborg M;Liu L;Dong M;Jensen HM;Bertelsen K;Thorsen M;Tan T;Xu X;Guo Z
通讯作者：
Guo Z