Single-dye-doped fluorescent nanoprobe enables self-referenced ratiometric imaging of hypochlorous acid in lysosomes
Single-dye-doped fluorescent nanoprobe enables self-referenced ratiometric imaging of hypochlorous acid in lysosomes
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单染料掺杂荧光纳米探针可实现溶酶体中次氯酸的自参考比率成像
DOI：
10.1016/j.snb.2019.127299
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发表时间：
2020-02
期刊：
影响因子：
--
通讯作者：
Chen Jian
中科院分区：
文献类型：
--
作者：
Ren Junyu;Zhang Peisheng;Liu Hui;Zhang Chonghua;Gao Yong;Cui Jiaxi;Chen Jian
The design of novel ratiometric nanoprobes with two well-resolved fluorescence bands for accurate imaging of lysosomal hypochlorous acid (HClO) is significant to understand its biological and pathological behaviors. Herein, we develop a single-dye-doped self-referenced ratiometric nanoprobe (SSRN) for imaging of HClO in lysosomes under a single-wavelength excitation (λex= 405 nm). The referenced unit (benzothiazole moiety) and HClO-responsive unit (rhodamine B) are integrated into the single dye (HB-Py) to achieve the self-referenced ratiometric effect. The well-dispersedSSRNare prepared by a simple coprecipitation of the morpholine moiety modified amphiphilic polymers (CO-720-MA) and HB-Py. Of note, the two well-resolved fluorescence bands (∼130 nm) ofSSRNcould provide accurate imaging of analyte in live cells. Additionally, other distinct merits with SSRN are also simultaneously realized, including high water solubility, high sensitivity (detection limit: 52 nM), excellent selectivity, good biocompatibility and excellent photostability. Significantly, benefiting from the morpholine moiety, this nanoprobeSSRNcan specially locate lysosomes and enable ratiometric imaging of exogenous/endogenous HClO in lysosomes.
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影响因子：
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作者：
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DOI：
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期刊：
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影响因子：
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