Deep Sequencing Transcriptome Analysis of Murine Wound Healing: Effects of a Multicomponent, Multitarget Natural Product Therapy-Tr14.
Deep Sequencing Transcriptome Analysis of Murine Wound Healing: Effects of a Multicomponent, Multitarget Natural Product Therapy-Tr14.
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鼠伤口愈合的深层测序转录组分析：多白素天然产品治疗-TR14的效果。
DOI：
10.3389/fmolb.2017.00057
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发表时间：
2017
影响因子：
5
通讯作者：
McCaffrey TA
中科院分区：
文献类型：
--
作者：
St Laurent G 3rd;Seilheimer B;Tackett M;Zhou J;Shtokalo D;Vyatkin Y;Ri M;Toma I;Jones D;McCaffrey TA
创面愈合是一个协调的反应过程，涉及多个过程，如止血、细胞迁移、细胞外基质合成，特别是炎症。利用小鼠皮肤伤口修复模型，绘制了损伤后12小时至8天的转录组图，并对多组分、多靶点天然产物Tr14做出了反应。利用单分子RNA测序（RNA-seq），发现与伤口愈合途径相关的已知转录本以及编码和非编码基因的新转录本存在明显的时间变化。Tr14处理可调节bbb100转录本，这些转录本与关键的伤口修复途径相关，如对损伤的反应、伤口收缩和细胞因子反应。这些结果提供了迄今为止转录组对皮肤损伤、修复和多组分天然产物治疗的反应的最精确和全面的表征。通过了解伤口修复过程和天然产物的作用，应该可以更有效地干预涉及异常修复的疾病。
Wound healing involves an orchestrated response that engages multiple processes, such as hemostasis, cellular migration, extracellular matrix synthesis, and in particular, inflammation. Using a murine model of cutaneous wound repair, the transcriptome was mapped from 12 h to 8 days post-injury, and in response to a multicomponent, multi-target natural product, Tr14. Using single-molecule RNA sequencing (RNA-seq), there were clear temporal changes in known transcripts related to wound healing pathways, and additional novel transcripts of both coding and non-coding genes. Tr14 treatment modulated >100 transcripts related to key wound repair pathways, such as response to wounding, wound contraction, and cytokine response. The results provide the most precise and comprehensive characterization to date of the transcriptome's response to skin damage, repair, and multicomponent natural product therapy. By understanding the wound repair process, and the effects of natural products, it should be possible to intervene more effectively in diseases involving aberrant repair.
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