Photoacoustic treatment mitigates cognitive dysfunction in a model of sleep-wake rhythm disturbance
Photoacoustic treatment mitigates cognitive dysfunction in a model of sleep-wake rhythm disturbance
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光声治疗可减轻睡眠-觉醒节律紊乱模型中的认知功能障碍
DOI：
10.4103/1673-5374.270415
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发表时间：
2019-12
影响因子：
6.1
通讯作者：
Xia Tianjiao
中科院分区：
文献类型：
--
作者：
Xing Fang;Fang Xin;Gong Xiangdan;Zhao Xin;Du Ying;Ma Zhengliang;Gu Xiaoping;Xia Tianjiao
以睡眠时间或持续时间异常为特征的睡眠-觉醒节律障碍与认知功能障碍有关。在异常睡眠-觉醒模式实验中，光声治疗包括光和声刺激在调节睡眠模式和改善认知行为方面被发现是有效的。在这项研究中，我们研究了光和声音干预是否可以减少睡眠节律障碍模型中的睡眠-觉醒模式障碍和记忆缺陷。采用人工敲笼、挤笼、扰巢等剥夺睡眠方法，建立C57BL/6J小鼠睡眠节律障碍模型。我们使用Mini Mitter无线电发送设备来监测小鼠的运动活动，并通过恐惧条件反射测试来评估认知功能。我们的研究结果表明，在小鼠主观上白天照射1小时的蓝光（40hz闪烁频率）可显著改善24小时顶相移，降低睡眠剥夺引起的记忆缺陷程度。然而，在偏移时间或主观夜间时间点暴露于40 hz蓝光，以及在3个干预时间点（主观白天时间、主观夜间时间和偏移时间点）暴露于2 hz蓝光的干预措施，对昼夜节律改变或记忆缺陷没有积极影响。此外，在主观白天时间，2000-Hz的声音干预可以减弱24小时的顶相移和记忆衰退，而440-Hz和4000-Hz的声音干预对昼夜节律没有影响。总之，这些结果表明，光声治疗有效地纠正了与睡眠剥夺引起的睡眠-觉醒节律紊乱相关的异常睡眠-觉醒模式和认知功能障碍。所有动物实验已于2017年11月20日获得中国南京大学医学院附属鼓楼医院实验动物伦理委员会批准（批准号20171102）。
Sleep-wake rhythm disturbances, which are characterized by abnormal sleep timing or duration, are associated with cognitive dysfunction. Photoacoustic treatments including light and sound stimulation have been found to be effective in modulating sleep patterns and improving cognitive behavior in abnormal sleep-wake pattern experiments. In this study, we examined whether light and sound interventions could reduce sleep-wake pattern disturbances and memory deficits in a sleep rhythm disturbance model. We established a model of sleep rhythm disturbance in C57BL/6J mice via a sleep deprivation method involving manual cage tapping, cage jostling, and nest disturbance. We used a Mini Mitter radio transmitter device to monitor motor activity in the mice and fear conditioning tests to assess cognitive function. Our results indicated that an intervention in which the mice were exposed to blue light (40-Hz flickering frequency) for 1 hour during their subjective daytime significantly improved the 24-hour-acrophase shift and reduced the degree of memory deficit induced by sleep deprivation. However, interventions in which the mice were exposed to a 40-Hz blue light at offset time or subjective night time points, as well as 2 Hz-blue light at 3 intervention time points (subjective day time, subjective night time, and offset time points), had no positive effects on circadian rhythm shift or memory deficits. Additionally, a 2000-Hz sound intervention during subjective day time attenuated the 24-hour-acrophase shift and memory decline, while 440-Hz and 4000-Hz sounds had no effect on circadian rhythms. Overall, these results demonstrate that photoacoustic treatment effectively corrected abnormal sleep-wake patterns and cognitive dysfunction associated with sleep-deprivation-induced disturbances in sleep-wake rhythm. All animal experiments were approved by the Experimental Animal Ethics Committee of Drum Tower Hospital Affiliated to the Medical College of Nanjing University, China (approval No. 20171102) on November 20, 2017.
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DOI：
10.1126/science.1180962
发表时间：
2009-11-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Kang JE;Lim MM;Bateman RJ;Lee JJ;Smyth LP;Cirrito JR;Fujiki N;Nishino S;Holtzman DM
通讯作者：
Holtzman DM
影响因子：
3.7
作者：
Kumar T;Jha SK
通讯作者：
Jha SK
影响因子：
3.5
作者：
Solenne Van der Maren;Christophe Moderie;C. Duclos;J. Paquet;Véronique Daneault;M. Dumont
通讯作者：
Solenne Van der Maren;Christophe Moderie;C. Duclos;J. Paquet;Véronique Daneault;M. Dumont
影响因子：
16.2
作者：
Ngo, Hong-Viet V.;Martinetz, Thomas;Moelle, Matthias
通讯作者：
Moelle, Matthias
影响因子：
2.3
作者：
Bersani, Giuseppe;Marconi, Daniela;Caroti, Eleonora
通讯作者：
Caroti, Eleonora