Sleep deprivation impairs consolidation of cued fear memory in rats.
Sleep deprivation impairs consolidation of cued fear memory in rats.
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DOI：
10.1371/journal.pone.0047042
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jha SK
中科院分区：
文献类型：
--
作者：
Kumar T;Jha SK
学习后的睡眠有助于负面记忆的巩固，并有助于将其保存数年。因此，人们相信，睡眠剥夺可能有助于防止恐惧记忆的巩固。然而，它对巩固负面/恐惧记忆的影响尚不清楚。提示恐惧条件反射（CuFC）是一个广泛使用的模型，以了解与焦虑症相关的负面记忆的神经基础。在这项研究中，我们首先确定了巩固CuFC记忆和CuFC后睡眠结构变化的合适昼夜节律时间。此后，我们研究了睡眠剥夺对CuFC记忆巩固的影响。在雄性Wistar大鼠（n = 51）中进行三组实验。 在实验I中，动物通过在开灯（7 AM）（n = 9）和关灯（7 PM）（n = 9）期间呈现10个音调-电击配对刺激来条件化线索恐惧。 在实验II中，动物准备多导睡眠图记录（n = 8）和睡眠结构的变化后，CuFC进行了测定。 进一步在实验III中，动物在熄灯期间被暗示恐惧条件反射，并被随机分为四组：睡眠剥夺（SD）（n = 9）、非睡眠剥夺（NSD）（n = 9）、压力控制（SC）（n = 9）和音调控制（n = 7）。 冷冻量的百分比，恐惧的标志，在这些组中进行了统计学比较。在关灯期间训练的大鼠表现出比开灯期间明显更多的冻结。在CuFC训练的动物中，总睡眠量没有变化，但是REM睡眠显著减少。此外，在总的睡眠时间中，动物在NREM睡眠中花费的时间比例更高。然而，与NSD和SC组相比，SD动物表现出显著更少的冻结。这些数据表明，睡眠在巩固线索恐惧条件记忆中起着重要作用。
Post-learning sleep facilitates negative memory consolidation and also helps preserve it over several years. It is believed, therefore, that sleep deprivation may help prevent consolidation of fearful memory. Its effect, however, on consolidation of negative/frightening memories is not known. Cued fear-conditioning (CuFC) is a widely used model to understand the neural basis of negative memory associated with anxiety disorders. In this study, we first determined the suitable circadian timing for consolidation of CuFC memory and changes in sleep architecture after CuFC. Thereafter, we studied the effect of sleep deprivation on CuFC memory consolidation. Three sets of experiments were performed in male Wistar rat (n = 51). In experiment-I, animals were conditioned to cued-fear by presenting ten tone-shock paired stimuli during lights-on (7 AM) (n = 9) and lights-off (7 PM) (n = 9) periods. In experiment-II, animals were prepared for polysomnographic recording (n = 8) and changes in sleep architecture after CuFC was determined. Further in experiment-III, animals were cued fear-conditioned during the lights-off period and were randomly divided into four groups: Sleep-Deprived (SD) (n = 9), Non-Sleep Deprived (NSD) (n = 9), Stress Control (SC) (n = 9) and Tone Control (n = 7). Percent freezing amount, a hallmark of fear, was compared statistically in these groups. Rats trained during the lights-off period exhibited significantly more freezing compared to lights-on period. In CuFC trained animals, total sleep amount did not change, however, REM sleep decreased significantly. Further, out of total sleep time, animals spent proportionately more time in NREM sleep. Nevertheless, SD animals exhibited significantly less freezing compared to NSD and SC groups. These data suggest that sleep plays an important role in the consolidation of cued fear-conditioned memory.
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DOI：
10.1038/nrn2881
发表时间：
2010-08
期刊：
Nature reviews. Neuroscience
影响因子：
--
作者：
Gerstner JR;Yin JC
通讯作者：
Yin JC
影响因子：
5.7
作者：
Knight DC;Waters NS;Bandettini PA
通讯作者：
Bandettini PA
影响因子：
3.4
作者：
Ishikawa, Akinori;Kanayama, Yasuyo;Nakamura, Shoji
通讯作者：
Nakamura, Shoji
影响因子：
5.3
作者：
Jha, SK;Jones, BE;Frank, MG
通讯作者：
Frank, MG
影响因子：
10.6
作者：
Backhaus, Jutta;Junghanns, Klaus;Hohagen, Fritz
通讯作者：
Hohagen, Fritz