The role of Ca2+ uptake in the response of human platelets to adrenaline and to 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor).
The role of Ca2+ uptake in the response of human platelets to adrenaline and to 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor).
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Ca2 摄取在人血小板对肾上腺素和 1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（血小板激活因子）反应中的作用。
DOI：
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
M. Scrutton
中科院分区：
文献类型：
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作者：
Katherine A. Clare;M. Scrutton
凝血酶诱导Ca2+摄取到搅拌和未搅拌的人血小板在存在或不存在乙酰水杨酸。这种Ca2+摄取与腺嘌呤核苷酸分泌密切相关，这与先前的观察结果一致[Massini， P.和l<s:1> scher， E.F. (1974) Biochim]。Biophys。Acta 372, 109-121]但在没有显著Ca2+摄取的情况下，观察到低水平的分泌。在乙酰水杨酸存在和不存在的情况下，1- o-烷基-2-乙酰- asn -甘油-3-磷酸胆碱（1-O-alkylAcGEPC）也诱导Ca2+摄取到搅拌和未搅拌的人血小板。在不添加纤维蛋白原的情况下，可以观察到1-O-alkylAcGEPC诱导的聚集和腺嘌呤核苷酸分泌，但添加纤维蛋白原导致1-O-alkylAcGEPC诱导的聚集和Ca2+摄取的剂量/反应曲线非常明显地向左移动。在没有添加纤维蛋白原的情况下，观察到Ca2+摄取与1- o -烷基lacgepc诱导的腺嘌呤核苷酸分泌密切相关。在添加纤维蛋白原的情况下，在没有检测到Ca2+摄取的情况下，可以观察到显著的聚集。肾上腺素诱导Ca2+摄取仅在加入纤维蛋白原的情况下进入搅拌的人血小板。阻断分泌，例如通过添加乙酰水杨酸，也阻止Ca2+摄取。在添加凝血酶观察到这种反应的条件下，添加肾上腺素不能引起乙酰水杨酸处理的血小板中磷脂酰肌醇或磷脂酰胆碱的分解。我们得出的结论是，凝血酶、1- o -烷基lacgepc和肾上腺素诱导的Ca2+摄取进入人血小板与分泌反应密切相关，在某些情况下，例如凝血酶的刺激，显然是后一种反应的结果。先前有报道称Ca2+摄取是人类血小板对肾上腺素反应的启动事件[Owen， n.e., Feinberg， H. and Le Breton， G.C. (1981) Am J. Physiol. 239, H483-488]，但在本研究中没有得到证实。
Thrombin induces Ca2+ uptake into both stirred and unstirred human platelets in the presence or absence of acetylsalicylate. This Ca2+ uptake is closely correlated with adenine nucleotide secretion in accord with previous observations [Massini, P. and Lüscher, E.F. (1974) Biochim. Biophys. Acta 372, 109-121] but a low level of secretion is observed in the absence of significant Ca2+ uptake. 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (1-O-alkylAcGEPC) also induces Ca2+ uptake into both stirred and unstirred human platelets in the presence and absence of acetylsalicylate. Aggregation and adenine nucleotide secretion induced by 1-O-alkylAcGEPC can be observed in the absence of added fibrinogen, but addition of fibrinogen causes a very marked shift to the left in the dose/response curves for aggregation and Ca2+ uptake induced by 1-O-alkylAcGEPC. In the absence of added fibrinogen a close correlation is observed between Ca2+ uptake and adenine nucleotide secretion induced by 1-O-alkylAcGEPC. In the presence of added fibrinogen significant aggregation can be observed in the absence of detectable Ca2+ uptake. Adrenaline induces Ca2+ uptake only into stirred human platelets in the presence of added fibrinogen. Blockade of secretion, e.g. by addition of acetylsalicylate, also prevents Ca2+ uptake. Addition of adrenaline fails to cause breakdown of phosphatidylinositol or phosphatidylcholine in acetylsalicylate-treated platelets under conditions where such a response is observed on addition of thrombin. We conclude that Ca2+ uptake into human platelets induced by thrombin, 1-O-alkylAcGEPC and adrenaline is closely associated with the secretory response and in some circumstances, e.g. stimulation by thrombin, is clearly a consequence of this latter response. Previous reports of Ca2+ uptake as an initiating event in the response of human platelets to adrenaline [Owen, N.E., Feinberg, H. and Le Breton, G.C. (1981) Am J. Physiol. 239, H483-488] have not been confirmed in this study.
1-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（血小板激活因子）刺激兔血小板中的钙流入。
DOI：
10.1016/s0006-291x(81)80147-7
发表时间：
1981
影响因子：
3.1
作者：
Lee,TC;Malone,B;Blank,ML;Snyder,F
通讯作者：
Snyder,F