Induced pluripotent stem cells for regenerative medicine.

Induced pluripotent stem cells for regenerative medicine.
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DOI:
10.1146/annurev-bioeng-071813-105108
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发表时间:
2014-07-11
影响因子:
9.7
通讯作者:
Roy K
Roy K
中科院分区:
工程技术1区
文献类型:
--
作者:
Hirschi KK;Li S;Roy K

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随着诱导多能干(iPS)细胞的发现,现在可以将分化的体细胞转化为具有产生成体组织所有细胞类型能力的多能干细胞。因此,该技术有广泛的应用,包括再生医学、体外疾病模型和药物筛选/发现。尽管细胞重编程的生物和生化技术已经成熟,但生物工程技术为 iPS 细胞的重编程、扩增、分离和分化提供了新的工具。在本文中,我们回顾了这些用于 iPS 细胞的衍生和操作的生物工程方法,并重点关注它们与再生医学的相关性。
With the discovery of induced pluripotent stem (iPS) cells, it is now possible to convert differentiated somatic cells into multipotent stem cells that have the capacity to generate all cell types of adult tissues. Thus, there is a wide variety of applications for this technology, including regenerative medicine, in vitro disease modeling, and drug screening/discovery. Although biological and biochemical techniques have been well established for cell reprogramming, bioengineering technologies offer novel tools for the reprogramming, expansion, isolation, and differentiation of iPS cells. In this article, we review these bioengineering approaches for the derivation and manipulation of iPS cells and focus on their relevance to regenerative medicine.
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