Sub-5-minute Detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR
Sub-5-minute Detection of SARS-CoV-2 RNA using a Reverse Transcriptase-Free Exponential Amplification Reaction, RTF-EXPAR
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使用无逆转录酶指数扩增反应 RTF-EXPAR 在 5 分钟内检测 SARS-CoV-2 RNA
DOI:
10.1101/2020.12.31.20248236
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发表时间:
2021
期刊:
影响因子:
--
通讯作者:
Carter J
中科院分区:
文献类型:
--
作者:
Carter J
We report a rapid isothermal method for detecting SARS-CoV-2, the virus responsible for COVID-19. The procedure uses a novel reverse transcriptase-free (RTF) approach for converting RNA into DNA, which triggers a rapid amplification using the Exponential Amplification Reaction (EXPAR). Deploying the RNA-to-DNA conversion and amplification stages of the RTF-EXPAR assay in a single step results in the detection of a sample of patient SARS-CoV-2 RNA in under 5 minutes.
DOI:
10.1073/pnas.2011221117
发表时间:
2020-09-29
影响因子:
11.1
作者:
Rabe BA;Cepko C
通讯作者:
Cepko C
影响因子:
19
作者:
Corman, Victor M.;Landt, Olfert;Drosten, Christian
通讯作者:
Drosten, Christian