Overexpression of γ-glutamylcysteine synthetase gene from Caragana korshinskii decreases stomatal density and enhances drought tolerance.
Overexpression of γ-glutamylcysteine synthetase gene from Caragana korshinskii decreases stomatal density and enhances drought tolerance.
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柠条γ-谷氨酰半胱氨酸合成酶基因的过度表达降低了气孔密度并增强了耐旱性
DOI：
10.1186/s12870-021-03226-9
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发表时间：
2021-10-01
影响因子：
5.3
通讯作者：
Bai J
中科院分区：
文献类型：
--
作者：
Lu B;Luo X;Gong C;Bai J
γ-谷氨酰半胱氨酸合成酶（γ-ECS）是谷胱甘肽生物合成的限速酶，在植物逆境应答中起着关键作用。本研究利用PEG 6000诱导干旱胁迫诱导锦鸡儿（Caragana korshinskii）γ-ECS（Ckγ-ECS）基因的内源表达。科尔申斯基。利用C. Korshinskii。分离的γ-ECS。与野生型相比，Ckγ-ECS过表达植株的γ-ECS活性增强，气孔密度和孔径减小，相对含水量增加，失水量减少，丙二醛含量降低。气孔发育相关基因EPF 1的mRNA表达量增加，法马和STOMAGEN的mRNA表达量降低。此外，生长素相关信号基因AXR 3和ARF 5的表达上调。这些变化表明，转基因拟南芥提高了耐旱性，Ckγ-ECS可能通过生长素信号途径调节EPF 1和STOMAGEN的mRNA表达，从而在气孔发育中起负调控作用。在线版本包含补充材料，可通过10.1186/s12870-021-03226-9获得。
Gamma-glutamylcysteine synthetase (γ-ECS) is a rate-limiting enzyme in glutathione biosynthesis and plays a key role in plant stress responses. In this study, the endogenous expression of the Caragana korshinskii γ-ECS (Ckγ-ECS) gene was induced by PEG 6000-mediated drought stress in the leaves of C. korshinskii. and the Ckγ-ECS overexpressing transgenic Arabidopsis thaliana plants was constructed using the C. korshinskii. isolated γ-ECS. Compared with the wildtype, the Ckγ-ECS overexpressing plants enhanced the γ-ECS activity, reduced the stomatal density and aperture sizes; they also had higher relative water content, lower water loss, and lower malondialdehyde content. At the same time, the mRNA expression of stomatal development-related gene EPF1 was increased and FAMA and STOMAGEN were decreased. Besides, the expression of auxin-relative signaling genes AXR3 and ARF5 were upregulated. These changes suggest that transgenic Arabidopsis improved drought tolerance, and Ckγ-ECS may act as a negative regulator in stomatal development by regulating the mRNA expression of EPF1 and STOMAGEN through auxin signaling. The online version contains supplementary material available at 10.1186/s12870-021-03226-9.
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