Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere.
Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere.
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DOI：
10.1007/s11104-015-2389-5
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发表时间：
2015
期刊：
影响因子：
4.9
通讯作者：
Downie, J. Allan
中科院分区：
文献类型：
--
作者：
Garcia-Fraile, Paula;Seaman, Jonathan C.;Karunakaran, Ramakrishnan;Edwards, Anne;Poole, Philip S.;Downie, J. Allan
为了在豌豆根上形成固氮根瘤，豆类根瘤菌必须在根际具有竞争性。我们的目标是确定对根际健康很重要的基因。使用微阵列筛选标记了信号的突变体，以确定在豌豆根际生长减少的突变体。对候选突变体进行了对照评估，包括在微量培养基中的生长、在豌豆根际的生长以及在混合接种剂中对豌豆的侵染。突变基因经DNA测序鉴定，并经转导证实。在5508个带有签名标签的突变体中，微阵列显示有50个突变体的根际适合度降低。生长试验鉴定了6个具有根际特有表型的突变体。其中一个基因(AraE)的突变发生在一个阿拉伯糖分解代谢操纵子中，并阻止了阿拉伯糖的生长。另一个基因(PcaM)的突变，编码了原儿茶酸盐和羟基苯甲酸摄取的预测溶质结合蛋白，减少了原儿茶酸盐的生长。与混合接种剂相比，两个突变体对根瘤侵染的竞争性都降低了，但单独接种时豌豆正常结瘤。其他具有类似表型的突变被预测会影响次生代谢。豌豆根际阿拉伯糖和原儿茶酸的分解代谢对豌豆的竞争力具有重要意义。葡萄属植物。其他被预测参与次生代谢的基因也很重要。
To form nitrogen-fixing nodules on pea roots, Rhizobium leguminosarum biovar viciae must be competitive in the rhizosphere. Our aim was to identify genes important for rhizosphere fitness. Signature-tagged mutants were screened using microarrays to identify mutants reduced for growth in pea rhizospheres. Candidate mutants were assessed relative to controls for growth in minimal medium, growth in pea rhizospheres and for infection of peas in mixed inoculants. Mutated genes were identified by DNA sequencing and confirmed by transduction. Of 5508 signature-tagged mutants, microarrays implicated 50 as having decreased rhizosphere fitness. Growth tests identified six mutants with rhizosphere-specific phenotypes. The mutation in one of the genes (araE) was in an arabinose catabolism operon and blocked growth on arabinose. The mutation in another gene (pcaM), encoding a predicted solute binding protein for protocatechuate and hydroxybenzoate uptake, decreased growth on protocatechuate. Both mutants were decreased for nodule infection competitiveness with mixed inoculants, but nodulated peas normally when inoculated alone. Other mutants with similar phenotypes had mutations predicted to affect secondary metabolism. Catabolism of arabinose and protocatechuate in the pea rhizosphere is important for competitiveness of R.l. viciae. Other genes predicted to be involved in secondary metabolism are also important.
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影响因子：
4.4
作者：
MacLean, Allyson A.;MacPherson, Gordon;Finan, Turlough M.
通讯作者：
Finan, Turlough M.
影响因子：
2.2
作者：
Das, S;Noe, JC;Kitten, T
通讯作者：
Kitten, T
影响因子：
3.5
作者：
Gao, Mengsheng;Teplitslci, Max
通讯作者：
Teplitslci, Max
影响因子：
12.3
作者：
Silby MW;Cerdeño-Tárraga AM;Vernikos GS;Giddens SR;Jackson RW;Preston GM;Zhang XX;Moon CD;Gehrig SM;Godfrey SA;Knight CG;Malone JG;Robinson Z;Spiers AJ;Harris S;Challis GL;Yaxley AM;Harris D;Seeger K;Murphy L;Rutter S;Squares R;Quail MA;Saunders E;Mavromatis K;Brettin TS;Bentley SD;Hothersall J;Stephens E;Thomas CM;Parkhill J;Levy SB;Rainey PB;Thomson NR
通讯作者：
Thomson NR
DOI：
10.1099/00221287-87-2-343
发表时间：
1975-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
JOHNSTON, AWB;BERINGER, JE
通讯作者：
BERINGER, JE