Gene expression profile of the cartilage tissue spontaneously regenerated in vivo by using a novel double-network gel: comparisons with the normal articular cartilage.
Gene expression profile of the cartilage tissue spontaneously regenerated in vivo by using a novel double-network gel: comparisons with the normal articular cartilage.
复制标题
DOI：
10.1186/1471-2474-12-213
复制
发表时间：
2011-09-29
影响因子：
2.3
通讯作者：
Yasuda K
中科院分区：
文献类型：
--
作者：
Imabuchi R;Ohmiya Y;Kwon HJ;Onodera S;Kitamura N;Kurokawa T;Gong JP;Yasuda K
我们最近发现，在骨软骨缺损的底部植入由聚-（2-丙烯酰胺-2-甲基丙磺酸）和聚-（N， N'-二甲基丙烯酰胺）组成的双网（DN）水凝胶塞，可以诱导透明软骨样组织的自发再生。本研究的目的是澄清再生组织与正常关节软骨的基因表达谱。我们在兔股沟处制造了一个圆柱形骨软骨缺损。然后在缺损底部植入DN凝胶塞。术后2周和4周，采用DNA微阵列和免疫组化检查分析再生组织。再生组织的基因表达谱在宏观上与正常软骨相似，但存在一些细微差异。COL2A1、COL1A2、COL10A1、DCN、FMOD、SPARC、FLOD2、CHAD、CTGF、COMP基因在再生组织中的表达程度高于正常软骨。与正常软骨相比，再生组织中表达5倍及以上的前30个基因包括2型胶原、10型胶原、FN、vimentin、COMP、EF1alpha、TFCP2和GAPDH基因。使用DN凝胶再生的组织在基因上与关节软骨相似，但不完全相同。本研究中显示的遗传数据对未来研究确定参与自发软骨再生的特定基因是有用的。
We have recently found a phenomenon that spontaneous regeneration of a hyaline cartilage-like tissue can be induced in a large osteochondral defect by implanting a double-network (DN) hydrogel plug, which was composed of poly-(2-Acrylamido-2-methylpropanesulfonic acid) and poly-(N, N'-Dimetyl acrylamide), at the bottom of the defect. The purpose of this study was to clarify gene expression profile of the regenerated tissue in comparison with that of the normal articular cartilage. We created a cylindrical osteochondral defect in the rabbit femoral grooves. Then, we implanted the DN gel plug at the bottom of the defect. At 2 and 4 weeks after surgery, the regenerated tissue was analyzed using DNA microarray and immunohistochemical examinations. The gene expression profiles of the regenerated tissues were macroscopically similar to the normal cartilage, but showed some minor differences. The expression degree of COL2A1, COL1A2, COL10A1, DCN, FMOD, SPARC, FLOD2, CHAD, CTGF, and COMP genes was greater in the regenerated tissue than in the normal cartilage. The top 30 genes that expressed 5 times or more in the regenerated tissue as compared with the normal cartilage included type-2 collagen, type-10 collagen, FN, vimentin, COMP, EF1alpha, TFCP2, and GAPDH genes. The tissue regenerated by using the DN gel was genetically similar but not completely identical to articular cartilage. The genetic data shown in this study are useful for future studies to identify specific genes involved in spontaneous cartilage regeneration.
登录
查看更多内容
通过在具有不同电荷密度的合成水凝胶上培养来增强软骨形成 ATDC5 细胞的体外分化
影响因子：
9.7
作者：
Kwon, Hyuck Joon;Yasuda, Kazunori;Gong, Jian Ping
通讯作者：
Gong, Jian Ping
影响因子：
4.8
作者：
Månsson, B;Wenglén, C;Heinegård, D
通讯作者：
Heinegård, D
影响因子：
6.9
作者：
Blain, Emma J.;Gilbert, Sophie J.;Duance, Victor C.
通讯作者：
Duance, Victor C.
影响因子：
4.6
作者：
Ivkovic, S;Yoon, BS;Lyons, KM
通讯作者：
Lyons, KM
影响因子：
2.8
作者：
Driesang, IMK;Hunziker, EB
通讯作者：
Hunziker, EB