Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae.
Large-scale transcriptome comparison of sunflower genes responsive to Verticillium dahliae.
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对大丽黄萎病有反应的向日葵基因的大规模转录组比较
DOI：
10.1186/s12864-016-3386-7
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发表时间：
2017-01-06
期刊：
影响因子：
4.4
通讯作者：
Li Q
中科院分区：
文献类型：
--
作者：
Guo S;Zuo Y;Zhang Y;Wu C;Su W;Jin W;Yu H;An Y;Li Q
背景：向日葵黄萎病（SVW）是由大丽黄萎病（V. dahlia）根部感染引起的维管束病害。严重威胁向日葵的产量和品质。然而，控制这种疾病的化学和农艺措施并不有效。选择更具抗性的基因型是减少污染的理想策略。深入了解向日葵黄萎病的分子机制和遗传基础对于加速育种进程至关重要。结果：采用 RNA-Seq 方法对感染大丽花黄萎病的抗性 (S18) 和易感 (P77) 向日葵基因型的根进行全局转录组分析。在一段时间内（接种后 6、12 和 24 小时，以及 2、3、5 和 10 天）检查不同的成对转录组比较。在 RD、SD 和 D 数据集中，1231 个基因以基因型共同转录模式与 SVW 抗性相关。此外，在抗性和易感基因型中，分别有 759 个和 511 个基因与 SVW 抗性直接相关，且具有基因型特异性转录模式。大多数基因被证明参与植物防御反应；这些基因包括过氧化物酶 (POD)、谷胱甘肽过氧化物酶、水通道蛋白 PIP、几丁质酶、L-抗坏血酸氧化酶和 LRR 受体。对于抗性基因型中上调的基因型特异性差异表达基因（DEG），与易感基因型相比，在抗性基因型中观察到更高的平均倍数变化。在抗性基因型中下调的基因型特异性 DEG 中观察到相反的效应。 KEGG分析显示，植物激素信号转导类、植物-病原体相互作用类和类黄酮生物合成类分别有98个、112个和52个基因。其中许多基因，如 CNGC、RBOH、FLS2、JAZ、MYC2 NPR1 和 TGA，调节防御相关途径的关键点，可能有助于向日葵的大丽花弧菌抗性。结论：转录组分析结果为向日葵和大丽花弧菌之间的串扰相关的转录本提供了更清晰的了解。结果确定了几个差异表达的unigenes，这些unigenes涉及超敏反应（HR）和水杨酸（SA）/茉莉酸（JA）介导的抗大丽花弧菌的信号转导途径。这些结果可用于筛选抗性向日葵基因型。
Background:Sunflower Verticillium wilt (SVW) is a vascular disease caused by root infection with Verticillium dahliae (V. dahlia). It is a serious threat to the yield and quality of sunflower. However, chemical and agronomic measures for controlling this disease are not effective. The selection of more resistant genotypes is a desirable strategy to reduce contamination. A deeper knowledge of the molecular mechanisms and genetic basis underlying sunflower Verticillium wilt is necessary to accelerate breeding progress.Results:An RNA-Seq approach was used to perform global transcriptome profiling on the roots of resistant (S18) and susceptible (P77) sunflower genotypes infected with V. dahlia. Different pairwise transcriptome comparisons were examined over a time course (6, 12 and 24 h, and 2, 3, 5 and 10 d post inoculation). In RD, SD and D datasets, 1231 genes were associated with SVW resistance in a genotype-common transcriptional pattern. Moreover, 759 and 511 genes were directly related to SVW resistance in the resistant and susceptible genotypes, respectively, in a genotype-specific transcriptional pattern. Most of the genes were demonstrated to participate in plant defense responses; these genes included peroxidase (POD), glutathione peroxidase, aquaporin PIP, chitinase, L-ascorbate oxidase, and LRR receptors. For the up-regulated genotype-specific differentially expressed genes (DEGs) in the resistant genotype, higher average fold-changes were observed in the resistant genotype compared to those in the susceptible genotype. An inverse effect was observed in the down-regulated genotype-specific DEGs in the resistant genotype. KEGG analyses showed that 98, 112 and 52 genes were classified into plant hormone signal transduction, plant-pathogen interaction and flavonoid biosynthesis categories, respectively. Many of these genes, such as CNGC, RBOH, FLS2, JAZ, MYC2 NPR1 and TGA, regulate crucial points in defense-related pathway and may contribute to V. dahliae resistance in sunflower.Conclusions:The transcriptome profiling results provided a clearer understanding of the transcripts associated with the crosstalk between sunflower and V. dahliae. The results identified several differentially expressed unigenes involved in the hyper sensitive response (HR) and the salicylic acid (SA)/jasmonic acid (JA)-mediated signal transduction pathway for resistance against V. dahliae. These results are useful for screening resistant sunflower genotypes.
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