Rhythmic actomyosin-driven contractions induced by sperm entry predict mammalian embryo viability.
Rhythmic actomyosin-driven contractions induced by sperm entry predict mammalian embryo viability.
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DOI：
10.1038/ncomms1424
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发表时间：
2011-08-09
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
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受精诱导的细胞质流动是许多物种卵的保守特征。然而，到目前为止，细胞质流动对哺乳动物胚胎发育的重要性还不清楚。在这里，通过结合快速成像的新鲜受精的小鼠卵子与先进的图像分析的基础上粒子图像测速，我们表明，受精诱导有节奏的细胞质运动，与脉动的突起形成的精子头部上方。我们发现，这些运动是由受精引起的Ca2+振荡触发的肌动球蛋白细胞骨架的收缩引起的。最重要的是，运动和卵子激活事件之间的关系使得单独使用运动来预测受精卵的发育潜力成为可能。总之，这种方法提供了迄今为止最早，最快，非侵入性的方法来预测体外受精卵的活力，因此可以大大改善IVF治疗的前景。 在许多物种中，精子与卵子受精后，可以检测到细胞质流动细胞质物质的运动。在这项研究中，有节奏的细胞质流动被证明是诱导小鼠钙诱导的细胞骨架收缩，这可以用来预测受精的成功结果。
Fertilization-induced cytoplasmic flows are a conserved feature of eggs in many species. However, until now the importance of cytoplasmic flows for the development of mammalian embryos has been unknown. Here, by combining a rapid imaging of the freshly fertilized mouse egg with advanced image analysis based on particle image velocimetry, we show that fertilization induces rhythmical cytoplasmic movements that coincide with pulsations of the protrusion forming above the sperm head. We find that these movements are caused by contractions of the actomyosin cytoskeleton triggered by Ca2+ oscillations induced by fertilization. Most importantly, the relationship between the movements and the events of egg activation makes it possible to use the movements alone to predict developmental potential of the zygote. In conclusion, this method offers, thus far, the earliest and fastest, non-invasive way to predict the viability of eggs fertilized in vitro and therefore can potentially improve greatly the prospects for IVF treatment. Cytoplasmic flows—the movement of cytoplasmic material—can be detected following the fertilization of an egg by a sperm in many species. In this study, rhythmic cytoplasmic flows are shown to be induced in mice by calcium-induced cytoskeleton contractions which could be used to predict the successful outcome of fertilization.
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影响因子：
2.7
作者：
Meilhac, Sigolene M.;Adams, Richard J.;Morris, Samantha A.;Danckaert, Anne;Le Garrec, Jean-Francois;Zernicka-Goetz, Magdalena
通讯作者：
Zernicka-Goetz, Magdalena
影响因子：
11.8
作者：
Deng, Manqi;Suraneni, Praveen;Li, Rong
通讯作者：
Li, Rong
影响因子：
2.7
作者：
Markoulaki, S;Matson, S;Ducibella, T
通讯作者：
Ducibella, T
影响因子：
2.7
作者：
Ducibella, T;Huneau, D;Ozil, JP
通讯作者：
Ozil, JP
DOI：
10.2307/3314912
发表时间：
1987-09-01
影响因子：
0.6
作者：
LAWLESS, JF
通讯作者：
LAWLESS, JF