Factors influencing the induction of sister chromatid exchanges in mammalian cells by 12-O-tetradecanoyl-phorbol-13-acetate.
Factors influencing the induction of sister chromatid exchanges in mammalian cells by 12-O-tetradecanoyl-phorbol-13-acetate.
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影响 12-O-十四烷酰基-佛波醇-13-乙酸酯在哺乳动物细胞中诱导姐妹染色单体交换的因素。
DOI：
10.1093/carcin/2.7.601
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发表时间：
1981
期刊：
影响因子：
4.7
通讯作者：
Little,JB
中科院分区：
文献类型：
--
作者：
Nagasawa,H;Little,JB
The induction of sister chromatid exchanges (SCE) by 12-O-tetradecanoyl-phorbol-13-acetate (TPA) was measured in mouse IOT½ and 3T3 cells released from density inhibition, and Chinese hamster CHO cells synchronized by mitotic selection. The induced frequency of SCE was similar in each cell type, but depended upon the concentration of TPA employed, its source, and the particular lot from the same source. Most SCE occurred in the first two rounds of cell replication after addition of TPA. The induction of SCE by TPA was markedly suppressed if the fetal calf serum in the incubation medium was not heat-inactivated. The addition of superoxide dismutase to medium with heat-inactivated serum also suppressed the induction of SCE. These results suggest that free radicals, particularly the super-oxide anion, may be important intermediates in some of the biologic effects of TPA.
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DOI：
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发表时间：
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期刊：
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