Role of dietary Schizochytrium sp. in improving disease resistance of zebrafish through metabolic and microbial analysis
Role of dietary Schizochytrium sp. in improving disease resistance of zebrafish through metabolic and microbial analysis
复制标题
饮食中裂殖壶菌的作用。
DOI：
10.21203/rs.3.rs-88323/v1
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发表时间：
2020-10
期刊：
影响因子：
4.5
通讯作者：
Ning He
中科院分区：
文献类型：
--
作者：
Yanyan Shi;Xingyu Cao;Zhidong Ye;Yiyuan Xu;Yiming Wang;Zhipeng Li;Ning He
背景作为养殖鱼类的必需营养素，野生鱼类的鱼油已部分被植物成分替代，以防止过度捕捞。裂殖壶菌属 (Schizochytrium sp.) 是一种海洋微藻，被认为是鱼油的有前途的替代品，可改善鱼类的生长和脂肪酸组成。然而，对于补充微藻对鱼类的抗病性缺乏全面的认识。在本研究中，为了了解富含微藻的饮食的病原体抗性机制，饮食中裂殖壶菌的影响。研究了斑马鱼肠道微生物和代谢特征的影响。结果杀鱼爱德华氏菌攻击试验表明，120 g/kg裂殖壶菌属。补充补充剂可以显着提高斑马鱼的存活率。此外，在用裂殖壶菌喂养的斑马鱼中观察到更高的杯状细胞密度。体液代谢组学分析表明，添加裂殖壶菌的饮食。促进斑马鱼的 TCA 循环、能量供应、牛磺酸代谢和 L-丝氨酸代谢，同时降低胆固醇代谢。微生物组分析显示，120 g/kg Schizochytrium sp.补充饮食可以显着增加有益细菌（即乳杆菌、Dorea、丁酸球菌和假黄单胞菌）的丰度，并减少几种潜在的病原体（即黄杆菌、假单胞菌、柠檬酸杆菌和支原体）。联合组学分析表明，一些Dorea和Butyricicoccus物种可能是具有抗病性的候选益生菌。结论Schizochytrium sp.膳食补充剂。可以提高感染爱德华氏菌的斑马鱼的存活率。它进一步揭示了Schizochytrium sp。通过双组学分析，作为饲料添加剂有可能改善斑马鱼的新陈代谢和肠道健康，从而增强斑马鱼的抗病能力。我们的研究为发展对微藻喂养的水生动物抗病性的基本认识提供了新的见解。此外，该实验为筛选具有免疫调节特性的益生菌候选物提供了重要线索。
BackgroundAs an essential nutrient for farmed fish, the fish oil from wild fish has been partly replaced by vegetable ingredients to prevent overfishing. Schizochytrium sp., a type of marine microalgae, is considered as a promising alternative to fish oil for improving growth and fatty acid profile in fish. However, there lacks a comprehensive understanding of disease resistance on microalgae supplementation of fish. In the present study, to understand the pathogen-resistant mechanisms of diets enriched with microalgae, the effects of dietary Schizochytrium sp. on the intestine microbial and metabolic profile of zebrafish were investigated. ResultsThe challenge trial with Edwardsiella piscicida showed that 120 g/kg Schizochytrium sp. supplementation had a significantly higher survival rate of zebrafish. Additionally, higher goblet cell density was observed in zebrafish fed with Schizochytrium sp. Metabolomic analysis of humoral fluids indicated that the diet supplemented with Schizochytrium sp. boosted the TCA cycle, energy supply, taurine metabolism, and L-serine metabolism, whereas decreased cholesterol metabolism in zebrafish. The microbiome analysis revealed that a 120 g/kg Schizochytrium sp. supplemented diet could remarkably increase the abundance of beneficial bacteria (i.e., Lactobacillus, Dorea, Butyricicoccus, and Pseudoxanthomonas), and reduce several potential pathogens (i.e., Flavobacterium, Pseudomonas, Citrobacter, and Mycoplasmas). Combined omics analysis indicated that some Dorea and Butyricicoccus species might be candidate probiotics with disease resistance.ConclusionsDietary supplement of Schizochytrium sp. could improve the survival rate of zebrafish when infected with Edwardsiella piscicida. It further revealed that Schizochytrium sp. as feed additive had the potential to improve metabolism and the intestine health by dual-omics analysis, and thus enhance disease resistance of zebrafish. Our research provides a novel insight into developing the fundamental understanding of disease resistance in aquatic animals fed with microalgae. Moreover, this experiment shed substantial light on the screening of probiotic candidates with immunomodulatory properties.
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影响因子：
3.7
作者：
Sarker PK;Kapuscinski AR;Lanois AJ;Livesey ED;Bernhard KP;Coley ML
通讯作者：
Coley ML
影响因子：
2.8
作者：
N. Sood;P. K. Pradhan;Ravindra;D. K. Verma;M. Yadav;R. Mishra;U. Kumar;T. Swaminathan;N. Sood
通讯作者：
N. Sood;P. K. Pradhan;Ravindra;D. K. Verma;M. Yadav;R. Mishra;U. Kumar;T. Swaminathan;N. Sood
影响因子：
5.1
作者：
Shahrzad Badvipour;Everett Eustance;M. Sommerfeld
通讯作者：
Shahrzad Badvipour;Everett Eustance;M. Sommerfeld
增强的 TCA 循环可提高斑马鱼对溶藻弧菌感染的存活率
影响因子：
5.2
作者：
Yang MJ;Cheng ZX;Jiang M;Zeng ZH;Peng B;Peng XX;Li H
通讯作者：
Li H
影响因子：
4.5
作者：
Ganuza, E.;Benitez-Santana, T.;Izquierdo, M. S.
通讯作者：
Izquierdo, M. S.